Top Down Metabolic Control Analysis of Oxidative Phosphorylation at Different Rates in Potato Tuber Mitochondria
Mitochondria isolated from plant tissues have specific properties (such as exogenous NADH oxidation and the alternative oxidase) that may result in a distribution of control over oxidative phosphorylation that is different from the control in mitochondria from other sources. However relevant data are scarce1,2 and no complete description of the control of phosphorylation in plant mitochondria is available. This study is an attempt to establish the basis for the study of the regulation of mitochondrial function in the plant cell. As a model system we used mitochondria purified from potato tuber since they appear straightforward with respect to bioenergetic processes3,4.
KeywordsTitration Respiration Cyanide NADH Succinate
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- 4.P. Diolez and M.D. Brand, Proton fluxes during oxidative phosphorylation in potato mitochondria, in: “Molecular Biochemical and Physiological Aspects of Plant Respiration,” H. Lambers and L.H.W. van der Plas, eds, SPB Academic Publishing, The Hague, in press.Google Scholar