Top Down Metabolic Control Analysis of Oxidative Phosphorylation at Different Rates in Potato Tuber Mitochondria

  • Adolf Kesseler
  • Philippe Diolez
  • Klaus Brinkmann
  • Martin D. Brand


Mitochondria isolated from plant tissues have specific properties (such as exogenous NADH oxidation and the alternative oxidase) that may result in a distribution of control over oxidative phosphorylation that is different from the control in mitochondria from other sources. However relevant data are scarce1,2 and no complete description of the control of phosphorylation in plant mitochondria is available. This study is an attempt to establish the basis for the study of the regulation of mitochondrial function in the plant cell. As a model system we used mitochondria purified from potato tuber since they appear straightforward with respect to bioenergetic processes3,4.


Respiratory Chain Alternative Oxidase Plant Mitochondrion Proton Leak Control Coefficient 
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  1. 1.
    R.C. Padovan, LB. Dry and J.T. Wiskich, An analysis of the control of phosphorylation coupled respiration in isolated plant mitochondria, Plant Physiol. 90:928–933 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    A.L. Moore, I.B. Dry and J.T. Wiskich, Regulation of electron transport in plant mitochondria under state 4 conditions, Plant Physiol. 95:34–40 (1991).PubMedCrossRefGoogle Scholar
  3. 3.
    P. Diolez and F. Moreau, Correlation between ATP synthesis, membrane potential and oxidation rate in plant mitochondria, Biochim. Biophys. Acta 806:56 -63 (1985).CrossRefGoogle Scholar
  4. 4.
    P. Diolez and M.D. Brand, Proton fluxes during oxidative phosphorylation in potato mitochondria, in: “Molecular Biochemical and Physiological Aspects of Plant Respiration,” H. Lambers and L.H.W. van der Plas, eds, SPB Academic Publishing, The Hague, in press.Google Scholar
  5. 5.
    G.C. Brown, R.P. Hafner and M.D. Brand, A ‘top-down’ approach to the determination of control coefficients in metabolic control theory, Eur. J. Biochem. 188:321–325 (1990).PubMedCrossRefGoogle Scholar
  6. 6.
    A.L. Moore and J.N. Siedow, The regulation and nature of the cyanide resistant alternative oxidase of plant mitochondria, Biochim. Biophys. Acta 1059:121–140 (1991).PubMedCrossRefGoogle Scholar
  7. 7.
    I.B. Dry, A.L. Moore, D.A. Day and J.T. Wiskich, Regulation of alternative pathway activity in plant mitochondria: non-linear relationship between electron flux and the redox poise of the quinone pool, Arch. Biochem. Biophys. 273:148–157 (1989).PubMedCrossRefGoogle Scholar
  8. 8.
    D.A. Day, LB. Dry, K.L. Soole, J.T. Wiskich and A.L. Moore, Regulation of alternative pathway activity in plant mitochondria, Plant Physiol. 95:948–953 (1991).PubMedCrossRefGoogle Scholar
  9. 9.
    A. Kesseler, P. Diolez, K. Brinkmann and M.D. Brand, Characterisation of the control of respiration in potato tuber mitochondria using the ‘top-down’ approach of metabolic control analysis, Eur. J. Biochem. 210:775–784 (1992).PubMedCrossRefGoogle Scholar
  10. 10.
    R.P. Hafher, G.C. Brown and M.D. Brand, Analysis of the control of respiration rate, phosphorylation rate, proton leak rate and protonmotive force in isolated mitochondria using the ‘top-down’ approach of meta-bolic control theory, Eur. J. Biochem. 188:313–319 (1990).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Adolf Kesseler
    • 1
  • Philippe Diolez
    • 2
  • Klaus Brinkmann
    • 1
  • Martin D. Brand
    • 3
  1. 1.Botanisches Institut, Abteilung für Experimentelle OekologieUniversität BonnBonnGermany
  2. 2.Biochimie Fonctionnelle des Membranes VégétalesCNRSGif-sur-YvetteFrance
  3. 3.Dept. of BiochemistryUniversity of CambridgeCambridgeEngland

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