A Microtitre Plate Method for Measuring Biopterin with Cryopreserved Crithidia Fasciculata

  • Robert J. Leeming
  • S. Kate Hall
  • Helen Friday
  • Philip Hurley
  • Anne Green
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 338)

Summary

The assay of biopterin derivatives in dried blood spots is used by us in initial screening for inherited defects in tetrahydrobiopterin synthesis. The previously described method (1) required aseptic technique and microbiological facilities. The modification detailed here has the advantages of antibiotic cover, which overcomes these needs and microtitre plate technology allowing the incubation time to be halved with precision and accuracy retained. Data reduction facilities may be applied.

Keywords

Folic Acid Phenylalanine Thiamine Riboflavin Pyridoxine 

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References

  1. 1.
    R.J.Leeming, P.A.Barford,J.A.Blair and I.Smith .Arch. Dis. Childhood 59: 58–61 (1984)CrossRefGoogle Scholar
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    R.J.Leeming, S.K.Hall, I.M.Surplice and A.Green .J. Inher. Metab. Dis. 13: 883–887 (1990)PubMedCrossRefGoogle Scholar
  3. 3.
    R.J.Leeming , J.A.Blair,A.Green and D.N.Raine.Arch. Dis. Childhood 51: 771–777 (1976)CrossRefGoogle Scholar
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    I.M.Surplice, P.D.Griffiths, A.Green and R.J.Leeming.J. Inher. Metab. Dis. 13: 169–171 (1990)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Robert J. Leeming
    • 1
  • S. Kate Hall
    • 1
  • Helen Friday
    • 1
  • Philip Hurley
    • 1
  • Anne Green
    • 1
  1. 1.Department of Clinical ChemistryChildrens HospitalBirminghamUK

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