A Microtitre Plate Method for Measuring Biopterin with Cryopreserved Crithidia Fasciculata
The assay of biopterin derivatives in dried blood spots is used by us in initial screening for inherited defects in tetrahydrobiopterin synthesis. The previously described method (1) required aseptic technique and microbiological facilities. The modification detailed here has the advantages of antibiotic cover, which overcomes these needs and microtitre plate technology allowing the incubation time to be halved with precision and accuracy retained. Data reduction facilities may be applied.
KeywordsAssay Culture Medium Phenylalanine Concentration Dihydropteridine Reductase MICROTITRE Plate Method Synthesis Deficiency
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