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Enzymes For Synthesis of 10-Formyltetrahydrofolate in Plants: Characterization of a Monofunctional 10-Formyltetrahydrofolate Synthetase and Copurification of 5,10-Methylenetetrahydrofolate Dehydrogenase and 5,10-Methenyltetrahydrofolate Cyclohydrolase Activities

  • Edwin A. Cossins
  • Carole D. Kirk
  • Helena C. Imeson
  • Li-li Zheng
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 338)

Abstract

Plants require folates for the biosynthesis of purines, serine, methionine, formylmethionyl-tRNA and thymidylate (1). In leaves, mitochondrial folates mediate glycine cleavage and serine formation during photorespiration (2). Plants also have enzymes (1,3) that interconvert methylene-and formyltetrahydrofolates. Alternatively, these species generate 10-HCO-H4PteGlu from formate (1). Recent work from Rabinowitz’s laboratory (4,5) on the latter reaction in spinach leaves, identified a monofunctional 10-HCO-H4PteGlu synthetase protein whose primary structure is like the synthetase domain of the mammalian and yeast trifunctional Cl-THF synthase. Partial purification of the related dehydrogenase and cyclohydrolase activities suggested they may occur as a bifunctional complex in spinach leaves (4). However, it is not clear whether this structural organization is typical of higher plants in general or may reflect a possible chloroplastic origin.

Keywords

Spinach Leave Streptomycin Sulphate Synthetase Protein High Plant Tissue Trifunctional Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Edwin A. Cossins
    • 1
  • Carole D. Kirk
    • 1
  • Helena C. Imeson
    • 2
  • Li-li Zheng
    • 3
  1. 1.Department of BotanyUniversity of AlbertaEdmontonCanada
  2. 2.Department of BiologyUniversity of LethbridgeLethbridgeCanada
  3. 3.Department of HorticultureXiongyue Agricultural CollegeXiongyueThe People’s Republic of China

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