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The Chemokines pp 211-212 | Cite as

Interleukin 8 (IL-8) is Produced by the Bronchoalveolar Lavage Cell Population from Patients with Active but not Inactive Sarcoidosis

  • A. Millar
  • Z. Brown
  • M. Spiteri
  • J. Westwick
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 351)

Abstract

Pulmonary sarcoidosis results in fibrotic changes within the lungs in some patients but the mechanisms by which this occurs are poorly understood. Interleukin-8 (IL-8) is a potent chemoattractant cytokine which has been proposed as playing a role in the development of lung injury and subsequent fibrosis in other forms of pulmonary disease. We considered that IL-8 might have a similar role in pulmonary sarcoidosis. In order to investigate this, bronchoalveolar lavage was performed on three groups of subjects: 6 symptomatic patients with biopsy proven sarcoidosis prior to any treatment, 6 asymptomatic patients with biopsy proven sarcoidosis and no treatment for at least 6 months, and 6 normal volunteers. Whole cell populations from this lavage were cultured for 24 hours in serum free media. The resultant supernatants were assayed for the presence of IL-8 using a highly specific double ELISA. No IL-8 was detected in the cell culture supernatants of the normal volunteers nor the asymptomatic patients with sarcoidosis, but was present (11.93 ± 0.71 ng/ml, SD ± SEM) in the supernatants from those with active sarcoidosis. These findings suggest that IL-8 production may have a role in the acute inflammatory phase of sarcoidosis, although the mechanisms by which subsequent fibrosis develops remain unclear.

Keywords

Neutrophil Infiltration Pulmonary Sarcoidosis Postgraduate Medicine Subsequent Fibrosis Neutrophil Chemotactic Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • A. Millar
    • 1
  • Z. Brown
    • 1
  • M. Spiteri
    • 2
  • J. Westwick
    • 3
  1. 1.Schools of Postgraduate MedicineUK
  2. 2.Royal Free HospitalLondonUK
  3. 3.Pharmacy and PharmacologyUniversity of BathBathUK

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