Localizing Putative Excitatory Endings in the Cochlear Nucleus by Quantitative Immunocytochemistry
A major characteristic of CN neurons is their rich and complex patterns of synaptic inputs. The input carried by the auditory nerve (AN) is relayed to CN neurons through very secure excitatory synapses (reviewed by Wenthold and Martin,’ 84 and Caspary,’ 86, see also chapter by Morest in this volume). In addition, an intricate array of intrinsic and extrinsic (descending) connections from a variety of sources (see chapter by Saint-Marie et al. in this volume), make inhibitory as well as excitatory synapses on CN neurons. This pattern of synaptic inputs is specific for each cell type and determines in part the different processing abilities of CN neurons. Therefore, analyzing the synaptology of CN neurons is essential for an adequate understanding of the roles of CN in auditory processing. Several questions relevant to understand the functional synaptology of the CN are addressed in several chapters of this volume. Here we report on some of our recent findings regarding the chemical anatomy of putative excitatory endings in the CN.
KeywordsAlbumin Glycine Paraformaldehyde Glutaraldehyde Purpura
Unable to display preview. Download preview PDF.
- Caspary, D.M., 1986, Cochlear Nuclei: Functional neuropharmacology of the different cell types, in: “Neurobiology of Hearing: The Cochlea”, R.A. Altschuler, R.P. Bobbin, D.W. Hoffman, eds., pp. 303–332, Raven Press, New York.Google Scholar
- De Mey, R.J., 1983, The preparation of Immunoglobulin gold conjugates (IGS reagents) and their use as markers for light and electron microscopic immunocytochemistry, in: “Immunocytochemistry”,, A.C. Cuello, ed., pp. 347–372, John Willey and Sons, New York.Google Scholar
- Gray, E.G., 1969, Electron microscopy of excitatory and inhibitory synapses, “Mechanisms of synaptic transmission”, Progress in Brain Res., Vol. 37.Google Scholar
- Moore, JK., 1986, Cochlear nuclei: Relationship to the auditory nerve, in: “Neurobiology of Hearing: The Cochlea”, R.A. Altschuler, R.P. Bobbin, D.G. Hooffman eds., pp. 283–301, Raven Press, New York.Google Scholar
- Ottersen, O.P., Storm-Mathissen, J., Madsen, S., Skumlien, S. and Sromhaug, J., 1989, Evaluation of the immunocytochemical method for amino acids, Med. Biol., 64: 147–158.Google Scholar
- Pappas, G.D. and Waxman, S.G., 1972, Synaptic fine structure-morphological correlates of chemical and electrotonic transmission, in: “Structure and Function of Synapses”, G.D. Pappas, D.K. Purpura, eds., pp. 1–44, Raven Press, New York.Google Scholar
- Potashner, S.J., Morest, D.K., Oliver, D.L. and Jones, D.R., 1985, Identification of glutamatergic and aspartatergic pathways in the auditory pathway, in: “Auditory Biochemistry”, D.G. Drescher, ed., pp. 141–162, C. Thomas, Springfield.Google Scholar
- Somogyi, P., Hodgson, A.J., Chubb, I.W., Penke, B. and Erdei, A., 1985, Antisera to gamma-aminobutyric acid application to the central nervous system, J. Histochem. Cytochem., 33:240–248.Google Scholar
- Wenthold, R.J., 1985, Glutamate and aspartate as neurotransmitters of the auditory nerve, in: “Auditory Biochemistry”, D.G. Drescher, ed., pp. 125–140, C. Thomas, Springfield.Google Scholar