Comparison of Langerhans Cells and Interdigitating Reticulum Cells

  • Mikihiro Shamoto
  • Satoru Hosokawa
  • Masanori Shinzato
  • Chiyuki Kaneko
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)


In our previous paper (1, 2) we reported that both OKT-6 and S-100 protein positive cells were observed in superficial and hilar lymph nodes. However, in mesenteric lymph nodes and spleens, only S-100 protein positive and OKT-6 negative cells were found. For immunostaining using OKT-6 and S-100 protein antibodies, mirror sections were used. Immunoelectron microscopy were also undertaken. We concluded that both positive cells were Langerhans cells (LCs), but only S-100 protein positive cells were interdigitating reticulum cells (IDCs). We then tried the double staining method in order to obtain more exact results, namely to prove whether LCs might be positive for both OKT-6 and S-100 protein.


Mesenteric Lymph Node Hilar Lymph Node Methyl Benzoate Superficial Lymph Node Birbeck Granule 
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  1. 1.
    M. Shamoto, M. Shinzato, S. Hosokawa et al., Langerhans cells in the lymph nodes: mirror section and immunoelectron microscopic studies., Virchows Arch (B) 561: 337 (1992).CrossRefGoogle Scholar
  2. 2.
    M. Shamoto, M. Shinzato, The characterization of interdigitating reticulum cells: a comparison between Langerhans cells., in: Dendritic Cells in Lymphoid Tissues, Intern Congr Series 926, Excerpta Medica, Y. Imai, J.G. Tew, E.C.M. Hoefsmit, Eds., Amsterdam, pp. 65 (1991).Google Scholar
  3. 3.
    Y. Sato, K. Murai, S. Watanabe et al., The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining., Am J Pathol, 125: 431 (1986).PubMedGoogle Scholar
  4. 4.
    K. Hashimoto, W.M. Tarnowski, Some new aspects of the Langerhans cell., Arch Dermatol, 97: 450 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    A.S. Zelickson, J.H. Mottaz, Epidermal dendritic cells, a quantitative study., Arch Dermatol, 98: 652 (1968).PubMedCrossRefGoogle Scholar
  6. 6.
    S. Hosokawa, M. Shinzato, C. Kaneko, M. Shamoto, Studies on Langerhans cells in the tracheal squamous metaplasia of vitamin A deficient rats., 2nd Intern Symp on Dendritic Cells in Fundamental and Clinical Immunology, Amsterdam (1992).Google Scholar
  7. 7.
    R.M. Steinman, M.C. Nussenzweig, Dendritic cells: features and functions., Immunol Rev, 53: 127 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    G. Stingl, Tamaki, S.I. Katz, Origin and function of epidermal Langerhans cells., Immunol Rev, 53: 149 (1980).PubMedCrossRefGoogle Scholar
  9. 9.
    B.M. Balfour, H.A. Drexhage, E.W.A. Kamperdijk, E.C.M. Hoefsmit, Antigen presenting cells including Langerhans cells, veiled cells and interdigitating cells., Ciba Found Symp, 84: 281 (1981).PubMedGoogle Scholar
  10. 10.
    S.M. Breathnach, The Langerhans cells., Br J Dermatol, 119: 463 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Mikihiro Shamoto
    • 1
  • Satoru Hosokawa
    • 1
  • Masanori Shinzato
    • 1
  • Chiyuki Kaneko
    • 1
  1. 1.Division of Pathological CytologyFujita Health University School of MedicineToyoake, AichiJapan

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