Rat Thymic Dendritic Cells: Flow Cytometry Analysis

  • María Purificacion Bañuls
  • Alberto Alvarez
  • Isabel Ferrero
  • Agustín Zapata
  • Carlos Ardavin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

Thymic dendritic cells (DC) are considered to be involved in negative selection of T cells leading to the acquisition of self-tolerance1,2, and many lines of evidence suggest that they may participate in the maturational process of thymocyte development3, in the clonal amplification of mature medullary thymocytes4, and in intrathymic presentation of non-MHC antigens5,6. Besides, recent reports analyzing the cell surface markers of murine thymic DC7,8 have demonstrated that these cells express some interesting molecules, particularly the CD8 antigen. The expression of CD8 by thymic DC may promote a mechanism for elimination of self-reactive thymocytes, as Sambhara and Miller9 have demonstrated that clonal deletion can be triggered if T cells that have been signaled through their TCR, also receive a second signal through the α3 domain of their class I MHC molecules, which can be delivered by a CD8 molecule.

Keywords

EDTA Iodide Integrin OX17 Collagenase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    P. Matzinger and S. Guerder, Does T-cell tolerance require a dedicated antigen presenting cell?, Nature 338: 74 (1989).PubMedCrossRefGoogle Scholar
  2. 2.
    F. Ramsdell and B.J. Fowlkes, Clonal deletion versus clonal anergy: the role of the thymus in inducing self tolerance, Science 248: 1342 (1990).PubMedCrossRefGoogle Scholar
  3. 3.
    M. Lafontaine, D. Landry, N. Blanc-Brunât, M. Pelletier, and S. Montplaisir, IL-1 production by human thymic dendritic cells: studies on the interrelation with accessory function, Cell.Immunol. 135: 431 (1991).PubMedCrossRefGoogle Scholar
  4. 4.
    D. Landry, L. Doyon, J. Poudrier, M. Lafontaine, M. Pelletier, and S. Montplaisir, Accessory function of human thymic dendritic cells in ConA induced proliferation of autologous thymocyte subsets, J. Immunol. 1990. 144: 836.PubMedGoogle Scholar
  5. 5.
    B. A. Kyewski, C. G. Fathman, and R. V. Rouse, Intrathymic presentation of circulating non-MHC antigens by medullary dendritic cells, J. Exp. Med. 163: 231 (1986).PubMedCrossRefGoogle Scholar
  6. 6.
    M. Zöller, Intrathymic presentation by dendritic cells and macrophages: their role in selecting T cells with specificity for internal and external nominal antigen, Immunology 74: 407 (1991).PubMedGoogle Scholar
  7. 7.
    C. Ardavin and K. Shortman, Cell surface marker analysis of mouse thymic dendritic cells, Eur. J. Immunol 22: 859 (1992).PubMedCrossRefGoogle Scholar
  8. 8.
    D. Vremec, M. Zorbas, R. Scollay, D. Saunders, C. Ardavin, L. Wu, and K. Shortman, The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells, J. Exp. Med. In press.Google Scholar
  9. 9.
    S. R. Sambhara and R. G. Miller, Programmed cell death of T cells signaled by the T cell receptor and the α3 domain of class I MHC, Science 252: 1424 (1991).PubMedCrossRefGoogle Scholar
  10. 10.
    R. M. Steinman, The dendritic cell system and its role in immunogenicity, Annu. Rev. Immunol. 9: 271 (1991).PubMedCrossRefGoogle Scholar
  11. 11.
    D. Landry, M. Lafontaine, M. Cossette, H. Barthelemy, C. Chartrand, S. Montplaisir, and Pelletier, Human thymic dendritic cells. Characterization, isolation and functional assays, Immunology 65: 135 (1988).PubMedGoogle Scholar
  12. 12.
    P. S. Freudenthal and R. M. Steinman, The distinct surface of human blood dendritic cells, as observed after an improved isolation method, Proc. Natl. Acad. Sci. USA 87: 7698 (1990).PubMedCrossRefGoogle Scholar
  13. 13.
    M. Crowley, K. Inaba, M. Witmer-Pack, and R. M. Steinman, The cell surface of mouse dendritic cells: FACS analysis of dendritic cells from different tissues including thymus, Cell. Immunol. 118: 108 (1989).PubMedCrossRefGoogle Scholar
  14. 14.
    A. M. Pollard and M. F. Lipscomb, Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells, J. Exp. Med. 172: 159 (1990).PubMedCrossRefGoogle Scholar
  15. 15.
    G. C. MacPherson, Properties of lymph-borne (veiled) dendritic cells in culture I. modulation of phenotype, survival and function: partial dependence on GM-CSF, Immunology 68: 102 (1989).PubMedGoogle Scholar
  16. 16.
    G. A. van Seventer, Y. Shimizu, and S. Shaws, Roles of multiple accessory molecules in T-cell activation, Curr. Opin. Immunol 3: 294 (1991).PubMedCrossRefGoogle Scholar
  17. 17.
    J. G. Damoiseaux, E. A. Dopp, J. J. Neefjes, R. H. Beelen, and C.D. Dijkstra, J. Leucocyte Biol. 46: 556 (1989).Google Scholar
  18. 18.
    A. Bellacosa and P. A. Lazo, The rat leukocyte antigen MRC OX-44 is a member of a new family of cell surface proteins which appear to be involved in growth regulation, Mol. Cell. Biol. 11: 2864 (1191).Google Scholar
  19. 19.
    D. Landry, M. Lafontaine, H. Barthelemy, N. Paquette, C. Chartrand, M. Pelletier, and S. Montplaisir, Human thymic dendritic cell-thymocyte association: ultrastructural cell phenotype analysis, Eur. J. Immnunol. 19: 1855 (1989).CrossRefGoogle Scholar
  20. 20.
    M. T. Crowley, K. Inaba, M. D. Witmer-Pack, S. Gezelter, and R. M. Steinman, Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen, J. Immunol. Methods 133: 55 (1990).PubMedCrossRefGoogle Scholar
  21. 21.
    G. G. MacPherson, S. Fossum, and B. Harrison, Properties of lymph-borne (veiled) dendritic cells in culture II. Expression of the IL-2 receptor: role of GM-CSF, Immunology 68: 108 (1989).PubMedGoogle Scholar
  22. 22.
    S. M. Setum, J. R. Serie, and O. D. Hegre, Comparative analysis of potency of splenic dendritic and adherent cell (macrophages) as alloantigen presenters in vivo, Diabetes 40: 1719 (1991).PubMedCrossRefGoogle Scholar
  23. 23.
    R. Navarro, C. Ardavin, A. M. Fontecha, A. Alvarez, and A. Zapata, In vitro characterization of rat thymic macrophages, Immunology 73: 114 (1991).PubMedGoogle Scholar
  24. 24.
    C. L. Tucek and R. L. Boyd, Surface expression of CD4 and Thy-1 on mouse thymic stromal cells, Int. Immunol. 2: 593 (1990).PubMedCrossRefGoogle Scholar
  25. 25.
    H. He, P. Naquet, D. Caillot, and M. J. Pierres, Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2+-independent mechanism, J. Exp. Med. 1991. 173: 515.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • María Purificacion Bañuls
    • 1
  • Alberto Alvarez
    • 1
  • Isabel Ferrero
    • 1
  • Agustín Zapata
    • 1
  • Carlos Ardavin
    • 1
  1. 1.Department of Cell Biology Faculty of BiologyComplutense UniversityMadridSpain

Personalised recommendations