The Effect of Human Dendritic Cells on the Lectin-Induced Responsiveness of CD4+ T Cells To IL-2 and IL-4

  • Sergiusz Markowicz
  • Anita Mehta
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 329)

Abstract

Human peripheral blood dendritic cells (DC) are potent accessory cells (AC) for the lectin-induced proliferative response of T lymphocytes1. However, the contribution of DC to the responsiveness of lectin-induced T cells to IL-2 and IL-4 is not clearly understood. The AC-dependent induction of IL-2 responsiveness by mitogenic lectin has been reported2. It has been also shown that the proliferation of lectin-induced T cells is promoted bv IL-2 or IL-4 in the absence of AC.3

Keywords

Arthritis Concanavalin Gentamycin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    J. W. Young and R. M. Steinman, Accessory cell requirements for the mixed-leucocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells, Celllmmunol 111: 167 (1988).Google Scholar
  2. 2.
    T. Hunig, M. Loos, and A. Schlimpl. The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and interleukin 2 responsiveness by Concanavalin A are accessory cell dependent, Eur. J. Immunol 13: 1 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    R. J. Armitage, A. E. Namen, H. M. Sassenfeld, and K. H. Grabstein, Regulation of human T cell proliferation by IL-7, J. Immunol. 144: 938 (1990).PubMedGoogle Scholar
  4. 4.
    J. Lifson, A. Raubitschek, C. Benike, K. Koths, A. Ammann, P. Sondel, and E. G. Engleman, Purified interleukin-2 induces proliferation of fresh human lymphocytes in the absence of exogeneous stimuli, J. Biol. Response Mod. 5: 61 (1986).PubMedGoogle Scholar
  5. 5.
    B. K. Mookerje and J. L. Pauly, Human recombinant interleukin-2 is mitogenic to human lymphocytes, J. Leuk. Biol 38: 553 (1985).Google Scholar
  6. 6.
    H. Spits, H. Yssel, Y. Takebe, N. Arai, T. Yokota, F. Lee, K. Arai, J. Banchereau, and J. E. de Vries, Recombinant interleukin 4 promotes the growth of human T cells, J. Immunol. 139: 1142 (1987).PubMedGoogle Scholar
  7. 7.
    S. H. Smith, M. H. Brown, D. Rowe, R. E. Callard, and P. C. L. Beverley, Functional subsets of human helper inducer cells defined by a new monoclonal antibody, UCHL-1, Immunology 58: 63 (1986).PubMedGoogle Scholar
  8. 8.
    C. Huber, M. Merkenschlager, C. Gattringer, I. Royston, U. Fink, and H. Braunsteiner, Human autologous mixed reactivity is primarily specific for xenoprotein determinants absorbed to antigen-presenting cells during rosette formation with sheep erythrocytes, J. Exp. Med. 155: 1222 (1982).PubMedCrossRefGoogle Scholar
  9. 9.
    V. Tsai and N. J. Zvaifler, Dendritic cell-lymphocyte clusters that form spontaneously in rheumatoid arthritis synovial effusions differ from clusters formed in human mixed leucocyte reactions, J. Clin. Jnv. 82: 1731 (1988).Google Scholar
  10. 10.
    S. Markowicz and E. G. Engleman, Granulocyte-macrophage colony-stimulating factor promotes differentiation and survival of human peripheral blood dendritic cells in vitro, J. Clin. Inv. 85: 955 (1990).CrossRefGoogle Scholar
  11. 11.
    E. G. Engleman, C. J. Benike, F. C. Grumet, and R. L. Evans, Activation of human T lymphocyte subsets: helper and suppressor/cytotoxic cells recognize and respond to distinct histocompatibility antigens, J. Immunol. 127: 2124 (1981).PubMedGoogle Scholar
  12. 12.
    M. A. Valentine, C. D. Tsoukas, G. Rhodes, J. H. Vaughan, and D. A. Carson, Phytohemagglutinin binds to the 20-kDa molecules of the T3 complex, Eur. J. mmunol. 15: 851 (1985).CrossRefGoogle Scholar
  13. 13.
    K. O’Flynn, A.M. Krensky, P. C. L. Beverley, S. J. Burakoff, and D. C. Linch, Phytohemagglutinin activation of T cells through the sheep red blood cell receptor, Nature 313: 686 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    R. M. Steinman, Cytokines amplify the function of accessory cells, Immunology Letters 17: 197 (1988).PubMedCrossRefGoogle Scholar
  15. 15.
    N. K. Damle, K. Klussman, P. S. Linsley, and A. Aruffo, Differential costimulatory effects of adhesion molecules B7, ICAM-1, LFA-3, and VCAM-1 on resting and antigen-primed CD4+T lymphocytes, J. Immunol. 148: 1985 (1992).PubMedGoogle Scholar
  16. 16.
    J. A. Byrne, J. L. Butler, and M. D. Cooper, Differential activation requirements for virgin and memory T cells, J. Immunol. 141: 3249 (1988).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Sergiusz Markowicz
    • 1
  • Anita Mehta
    • 2
  1. 1.Department of ImmunologyThe Maria Sklodowska-Curie Memorial Cancer Center and Institute of OncologyWarsawPoland
  2. 2.Department of PathologyStanford University School of MedicineStanfordUSA

Personalised recommendations