Abstract
The mammalian blood-brain barrier (BBB) to non-lipid soluble macromolecules is associated morphologically with two basic cell types: the cerebral, non-fenestrated endothelium and perivascular phagocytes; the latter is represented by macrophages, microglia and pericytes surrounded by a basal lamina and located on the abluminal surfaces of cerebral arterioles, venules, and less so capillaries (1). Characteristics of the cerebral endothelium serving to define the BBB include circumferential belts of interendothelial tight junctional complexes that preclude the bidirectional, extracellular movement of macromolecules between blood and brain, and acid hydrolase-containing secondary lysosomes (4). The perivascular phagocytes also contain populations of secondary lysosomes and represent the first line of defense once the BBB is breached normally, experimentally, or pathologically (1, 10). In vitro and in vivo data implicate a third cell type, the astrocyte, in influencing the formation of interendothelial tight junctions; however, astrocytes arid their perivascular endfeet are not a physical or morphologically defined-barrier to the extracellular movement of macromolecules between the endothelium and brain parenchyma.
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References
Baker, B., Broadwell, R., and Wolf, A., Cellular line of defense upon breachment of the blood-brain barrier, Soc. Neurosci. Abstr., in press.
Balin, B.J., and Broadwell, R.D., Transcytosis of protein through the mammalian cerebral epithelium and endothelium. I. Choroid plexus and the blood-cerebrospinal fluid barrier, J. Neurocytol. 17: 809, 1988.
Banks, W.A., and Kastin, A.J., Review: Interactions between the blood-brain barrier and endogenous peptides: Emerging clinical implications, Amer.J. Med. Sci. 31: 459, 1988.
Broadwell, R.D., Transcytosis of macromolecules through the blood-brain barrier. A critical appraisal and cell biological perspective, Acta Neuropath. 79: 117, 1989.
Broadwell, R.D., Transcytosis of macromolecules through the fluid-brain barriers in vivo. “Biological Barriers to Protein Delivery,” Vol. 5, in press, 1992.
Broadwell, R.D., Baker, B J., Ebert, P.S., Hickey, W.F., and Villegas, J., Intracerebral grafting of solid tissues and cell suspensions: The blood-brain barrier and host immune response, Prog. Brain Res., in press, 1992a.
Broadwell, R., Baker, B., Friden, P., Tangoren, M., and Wolf, A., Receptor-mediated transcytosis through the blood-brain barrier Ferro-transferrin and its receptor, Soc. Neurosci. Abstr., in press, 1992b.
Broadwell, R.D., Balin, B J., Salcman, M., and Kaplan, R.S., A brain-blood barrier? Yes and no, Proc. Nat. Acad. Sci. USA 80: 7352, 1983.
Broadwell, R.D., Balin, B J., and Salcman, M., Transcytosis of blood-borne protein through the blood-brain barrier, Proc. Nat. Acad. Sci. USA 85: 632, 1988.
Broadwell, R.D., and Salcman, M., Expanding the definition of the blood-brain barrier, Proc. Natl. Acad. Sci. USA 78: 7820, 1981.
Broadwell, R.D., and Sofroniew, M.V., Serum proteins by-pass the blood-brain barrier for extracellular entry to the central nervous system, submitted toJ. Neurosci. 1992.
Palade, G., Intracellular aspects of the process of protein secretion, Science 189: 347, 1975.
Pardridge, W.M., “Peptide Drug Delivery to the Brain,” Raven Press, New York, 1991.
Villegas, J., and Broadwell, R.D., Transcytosis of protein through the mammalian cerebral epithelium and endothelium. II: Absorptive transcytosis and the blood-brain and brain-blood barriers, submitted toJ. Neurocytol.
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© 1993 Springer Science+Business Media New York
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Broadwell, R.D. (1993). Endothelial Cell Biology and the Enigma of Transcytosis Through the Blood-Brain Barrier. In: Drewes, L.R., Betz, A.L. (eds) Frontiers in Cerebral Vascular Biology. Advances in Experimental Medicine and Biology, vol 331. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2920-0_22
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DOI: https://doi.org/10.1007/978-1-4615-2920-0_22
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