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Plasmids as Testsystem for the Detection of DNA Strand Breaks

  • J. Wehner
  • G. Horneck
  • H. Bücker
Part of the NATO ASI Series book series (NSSA, volume 243A)

Abstract

The E. coli plasmid pUC19 was used to analyse the formation of strand breaks in the DNA. Strand breaks were induced by endonuclease DNAase I in the presence of Mg2+ or Mn2+ ions. Single as well as double strand breaks were analysed by gel electrophoresis. This testsystem will be used for detecting damage at the molecular level of DNA induced by radiation, especially by ionizing or vacuum-ultraviolet (V-UV) radiation.

Keywords

Strand Break Double Strand Break Subtilis Spore Cellular Repair High Copy Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • J. Wehner
    • 1
  • G. Horneck
    • 1
  • H. Bücker
    • 1
  1. 1.Biophysics DivisionDLR, Institute for Aerospace MedicineKöln 90Germany

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