Plasmids as Testsystem for the Detection of DNA Strand Breaks
The E. coli plasmid pUC19 was used to analyse the formation of strand breaks in the DNA. Strand breaks were induced by endonuclease DNAase I in the presence of Mg2+ or Mn2+ ions. Single as well as double strand breaks were analysed by gel electrophoresis. This testsystem will be used for detecting damage at the molecular level of DNA induced by radiation, especially by ionizing or vacuum-ultraviolet (V-UV) radiation.
KeywordsStrand Break Double Strand Break Subtilis Spore Cellular Repair High Copy Plasmid
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