Plasmids as Testsystem for the Detection of DNA Strand Breaks
The E. coli plasmid pUC19 was used to analyse the formation of strand breaks in the DNA. Strand breaks were induced by endonuclease DNAase I in the presence of Mg2+ or Mn2+ ions. Single as well as double strand breaks were analysed by gel electrophoresis. This testsystem will be used for detecting damage at the molecular level of DNA induced by radiation, especially by ionizing or vacuum-ultraviolet (V-UV) radiation.
KeywordsAgarose Bromide Electrophoresis MgCl2 Bacillus
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- Maezawa, H.; Ito, T.; Hieda, K.; Kobayashi, K.; Ito, A.; Mori, T.; Suzuki, K. 1984. Action Spectra for Inactivation of Dry Phage T1 after Monochromatic (150–254 nm) Synchrotron Irradiation in the Presence and Absence of Photoreactivation and Dark Repair. Radiation Research 98: 227–233.PubMedCrossRefGoogle Scholar