Abstract
The E. coli plasmid pUC19 was used to analyse the formation of strand breaks in the DNA. Strand breaks were induced by endonuclease DNAase I in the presence of Mg2+ or Mn2+ ions. Single as well as double strand breaks were analysed by gel electrophoresis. This testsystem will be used for detecting damage at the molecular level of DNA induced by radiation, especially by ionizing or vacuum-ultraviolet (V-UV) radiation.
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© 1993 Springer Science+Business Media New York
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Wehner, J., Horneck, G., Bücker, H. (1993). Plasmids as Testsystem for the Detection of DNA Strand Breaks. In: Swenberg, C.E., Horneck, G., Stassinopoulos, E.G. (eds) Biological Effects and Physics of Solar and Galactic Cosmic Radiation. NATO ASI Series, vol 243A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2918-7_4
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DOI: https://doi.org/10.1007/978-1-4615-2918-7_4
Publisher Name: Springer, Boston, MA
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