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Role of Elongation Factors in Steering the Ribosomal Elongation Cycle

  • Knud H. Nierhaus
  • Francisco Triana

Abstract

The detection and characterization of the third transfer RNA (tRNA)-binding site on the ribosome, the E site, in addition to the classical A and P sites has led to the allosteric three-site model (ref. 1 and references therein), which has provided fresh impetus for discussion of and insights into the ribosomal elongation mechanism. It allows, for example, for the first time the identification of a common inhibition mechanism for aminoglycoside antibiotics,2 which do not exert their antibiotic activity by inducing misreading3 as previously assumed. The implications of the allosteric three-site model for the selection of the correct aminoacyl-tRNA and for the role of the elongation factors will be surveyed here. We start with a brief description of the main features of the allosteric three-site model, then address the problem of recognition involved in the selection of cognate aminoacyl-tRNAs and describe a surprising solution to this problem, which might be related to such fundamental structural features as the two-subunit nature of all ribosomes. We close the chapter with a first attempt to describe the mechanism of both elongation factors.

Keywords

Elongation Factor Allosteric Transition Activation Energy Barrier Elongation Cycle Recognition Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Knud H. Nierhaus
    • 1
  • Francisco Triana
    • 1
  1. 1.Max-Planck-Institut für Molekulare GenetikBerlin 33Germany

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