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In Vitro Studies of Determinants of Smooth Muscle Mechanics

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Mechanism of Myofilament Sliding in Muscle Contraction

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 332))

Abstract

Smooth muscle contraction is dependent upon phosphorylation of the 20,000 Da light chain subunits of myosin. Whereas the kinetics of the hydrolysis of MgATP by smooth muscle myosin suggest a simple phosphorylation-dependent “on-off” mechanism, the contractile response in smooth muscle tissue is complex. Experiments to unravel this complexity have been performed in vitro using a combination of motility assays and kinetic techniques. Some insight into this complexity is obtained, but the mechanism and the regulation of smooth muscle contraction is still not completely known.

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Authors and Affiliations

  1. Laboratory of Molecular Cardiology National Heart, Lung, and Blood, Institute National Institutes of Health, Bethesda, MD, 20892, USA

    James R. Sellers, Seiji Umemoto & Giovanni Cuda

Authors
  1. James R. Sellers
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  2. Seiji Umemoto
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  3. Giovanni Cuda
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Editor information

Editors and Affiliations

  1. Teikyo University, Tokyo, Japan

    Haruo Sugi

  2. University of Washington, Seattle, Washington, USA

    Gerald H. Pollack

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© 1993 Springer Science+Business Media New York

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Sellers, J.R., Umemoto, S., Cuda, G. (1993). In Vitro Studies of Determinants of Smooth Muscle Mechanics. In: Sugi, H., Pollack, G.H. (eds) Mechanism of Myofilament Sliding in Muscle Contraction. Advances in Experimental Medicine and Biology, vol 332. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2872-2_26

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  • DOI: https://doi.org/10.1007/978-1-4615-2872-2_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6245-6

  • Online ISBN: 978-1-4615-2872-2

  • eBook Packages: Springer Book Archive

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