The Molecular Switch in Troponin C

  • John Gergely
  • Zenon Grabarek
  • Terence Tao
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 332)

Abstract

Conformational changes in troponin C (TnC) associated with Ca2+-induced triggering of muscle contraction are discussed in light of the model proposed by Herzberg, Moult and James (J. Biol. Chem. 261, , 2638, 1986) and of our recent work on mutants of troponin C. The model involves a Ca2+-induced angular movement of one pair of α-helical segments relative to another pair of helices in the N-terminal domain. A disulfide bridge introduced into the N-terminal domain reversibly blocks the key conformational transition and the Ca2+-regulatory activity. Binding of troponin I (TnI) to the disulfide form of TnC is weakened owing to the blocking of its interaction with the N-terminal domain; however incorporation of the mutant into TnC-extracted myofibrils is not abolished. Introduction of a Cys residue in the C-terminal domain of TnC leads to disulfide formation between it and the indigenous Cys-98, with accompanying inhibition of regulatory activity attributable to interference with binding to TnI and, consequently, incorporation into the thin filaments. Evidence for movement of helical segments upon Ca2+-binding to TnC was obtained by measurements of excimer fluorescence and of resonance energy transfer with probes attached to Cys residues introduced by site-directed mutagenesis at suitable locations. Introduction of a disulfide bridge into calmodulin, another member of the super-family of Ca2+-binding proteins to which TnC belongs, abolishes its interaction with target enzymes. This suggests that the type of conformational change involving angular movement of helical segments that takes place in TnC is also involved in signal transmission in other Ca2+-dependent regulatory proteins.

Keywords

Crystallization Turkey Disulfide Pyrene Clarification 

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • John Gergely
    • 1
    • 2
    • 3
  • Zenon Grabarek
    • 1
    • 2
    • 3
  • Terence Tao
    • 1
    • 2
    • 3
  1. 1.Department of Muscle ResearchBoston Biomedical Research InstituteBostonUSA
  2. 2.Dept. of NeurologyMassachusetts General HospitalBostonUSA
  3. 3.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA

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