The Role of Granulocytes, Naive and Activated Macrophages in the Host Resistance against Salmonella typhimurium
Mononuclear phagoytes play an important role in the innate resistance of mice against infection by Salmonella typhimurium. The outcome of an infection is influenced by a number of factors, ranging from the handling of the microorganisms by resident macrophages during the earliest phase of infection to the appearance of specific cellular and humoral immune responses in a later phase. Control of the rate of proliferation of S. typhimurium in the liver and spleen of mice during the first week of infection is the earliest process known to be genetically determined (Plant and Glynn, 1976; Hormeache, 1979; Hormeache, 1980a; Skamene et al., 1982). On the basis of the differences in the early in vivo outgrowth, inbred mice can be divided into resistant and susceptible strains. in vivo studies have indicated that the control of S. typhimurium proliferation is due to an inherent property of macrophages (Robson and Vas, 1972; Maier and Oels, 1972; Vas, 1978; O’Brien et al., 1979; Hormaeche et al., 1980b; O’Brien and Metcalf, 1982). Our studies showed no difference in the phagocytic ability of peritoneal macrophages from resistant CBA and susceptible C57BL/10 mice for S. typhimurium opsonized with immune serum, but we found that the initial rate of intracellular killing of ingested S. typhimurium by CBA macrophages was more rapid than by macrophages from C57BL/10 mice (Van Dissel et al., 1985), which reflects the constitutive difference between the two strains of mice. However, equal rates of intracellular killing of L. monocytogenes were found for macrophages from the Listeria-susceptible CBA mice and the Listeria-resistant C57BL/10 mice (Van Dissel et al., 1985), which indicates that other factors do well influence the outcome of infections with these bacteria. Such a factor could be the greater number of monocyte-derived exudate macrophages in the inflammatory exudate in C57BL/10 mice than in CBA mice (Stevenson et al., 1981; Sluiter et al., 1984), which is due to a difference in the response to the factor-increasing monocytopoiesis (Sluiter et al., 1984).
KeywordsPeritoneal Macrophage Inbred Mouse Purify Protein Derivative Resident Macrophage Natural Resistance
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