Abstract
The gene tlpA located outside the 8 kb virulence determinant region of the virulence plasmid of Salmonella enterica serovar Typhimurium has been cloned and sequenced. The sequence predicts a protein of 43 kDa with unique features for a Salmonella protein: extensive heptapeptide repeating structure, 80 % alphahelix, and an N-terminal globular portion. Sequence comparisons demonstrate strong homologies to the tropomyosin family of eukaryotic proteins; if so, it would be likely to occur as dimers with a coiled coil structure. Chemical and immunochemical data on the protein expressed in E. coli support these predictions.
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Mäkelä, P.H., Koski, P., Riikonen, P., Taira, S., Holthöfer, H., Rhen, M. (1993). The Virulence Plasmid of Salmonella Encodes a Protein Resembling Eukaryotic Tropomyosins. In: Cabello, F., Hormaeche, C., Mastroeni, P., Bonina, L. (eds) Biology of Salmonella . NATO ASI Series, vol 245. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2854-8_14
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DOI: https://doi.org/10.1007/978-1-4615-2854-8_14
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