Fluorescent Probes — Where Do they Go in Cells, and Why?

  • Richard W. Horobin
  • Fiza Rashid-Doubell


The fluorescent probes considered in this paper are either low molecular weight xenobiotics (e.g., rhodamine 123) or non-polymeric labelled metabolites (e.g., lipids), which are applied to living cells or organisms. Note that various other types of molecular probes, sometimes referred to as fluorescent probes, are excluded by this definition. Thus, macromolecular probes (e.g., fluorescent antibodies and lectins; nucleic acid probes) are not considered here. Neither are histochemical fluorochromes applied to fixed (i.e. dead) cells and tissues.


Fluorescent Probe Fluorescein Diacetate Magic Bullet Nucleic Acid Probe Cationic Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Horobin, R.W., and Rashid, F. (1990) Interactions of molecular probes with living cells and tissues. 1. Some general mechanistic proposals, making use of a simplistic Chinese box model. Histochemistry 94: 205.PubMedCrossRefGoogle Scholar
  2. Hansch, C, and Leo, A. (1979) “Substituent Constants for Correlation Analysis in Chemistry and Biology”, Wiley, Chichester.Google Scholar
  3. Rashid, F., and Horobin, R.W. (1990a) Interactions of molecular probes with living cells and tissues. 2. A structure-activity analysis of mitochondrial staining by cationic probes, and a discussion of the synergistic nature of image-based and biochemical approaches. Histochemistry 94: 303.PubMedCrossRefGoogle Scholar
  4. Rashid, F., and Horobin, R.W. (1990b) Physiochemical features of fluorescent nuclear probes — a basis for their rational selection? Proc. Roy. Microsc. Soc. 26: 58.Google Scholar
  5. Rashid, F., and Horobin, R.W. (1991) Accumulation of fluorescent non-cationic probes in mitochondria of cultured cells: observations, a proposed mechanism and some implications. J. Microsc. 163: 233.PubMedCrossRefGoogle Scholar
  6. Rashid, F., Horobin, R.W., and Williams, M.A. (1991) Predicting the behaviour and selectivity of fluorescent probes for lysosomes and related structures by means of structure-activity models. Histochemical J., 23: 450.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Richard W. Horobin
    • 1
  • Fiza Rashid-Doubell
    • 2
  1. 1.Department of Biomedical ScienceSheffield University SheffieldUK
  2. 2.Brain and Behaviour Research Group, Department of BiologyThe Open UniversityMilton KeynesUK

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