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Abstract

The fusion of secondary oocytes and blastomeres is a good tool for the study of the cellular events which occur during early embryo development. The secondary oocytes contain the maturation promoting factor and, therefore, it might be anticipated that the fusion of oocytes and interphase blastomeres would produce premature condensation of chromosomes (Murray, 1989; McConell, 1990).

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References

  • Bolton, V.N., Oades, P.J., and Johnson, M.H. (1984) The relationship between cleavage, DNA replication and gene expression in the mouse 2-cell embryo. J. Embryol. Exp. Morph. 79: 139–163.

    PubMed  CAS  Google Scholar 

  • Braude, P.R., Pelham, H., Flach, G., and Lobatto, R. (1979) Post-translational control in the early mouse embryo. Nature 282: 102–105.

    Article  PubMed  CAS  Google Scholar 

  • Flach, G., Johnson, M.H., Braude, P, R., Taylor, R.A.S., and Bolton, V.N. (1982) The transition from maternal to embryonic control in the 2-cell mouse embryo. EMBO J. 1: 681–686.

    PubMed  CAS  Google Scholar 

  • Fulton, B.P., and Whittingham, D.G. (1978) Activation of mammalian oocytes by intracellular injection of calcium. Nature 273: 149–151.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, M.H., McConell, J., and Van Blerkom, J. (1984) Programmed development in the mouse embryo. J. Embryol. Exp. Morph. 83: 197–231.

    PubMed  CAS  Google Scholar 

  • Johnson, M.H., and Pratt, H.P.M. (1983) Cytoplasmic localizations and cell interactions in the formation of the mouse blastocyst, in: “Time, Space and Pattern in Embryonic Development”, W.R. Jeffrey and R.A. Raff, eds., Alan R. Liss Inc., New York, 117–128.

    Google Scholar 

  • Kubiak, J.Z., Prather, R.S., Maul, G.G., and Schatten, G. (1991) Cytoplasmic modification of the nuclear lamina during pronuclear-like transformation of mouse blastomere nuclei. Mech. Develop. 35: 103–111.

    Article  CAS  Google Scholar 

  • Kubiak, J.Z., and Tarkowski, A.K. (1985) Electrofusion of mouse blastomeres. J. Embryol. exp. Morph. 18: 155–180.

    Google Scholar 

  • Murray, A.W. (1989) Cyclin synthesis and degradation in the embryonic cell cycle. J. Cell Sci. Suppl. 12: 65–76.

    PubMed  CAS  Google Scholar 

  • McConell, J. (1990) Cell cycle control in early mouse development. J. Reprod. Fertil. 42: 205–213.

    Google Scholar 

  • McLachlin, J.R., Cavaney, S., and Kidder, G.M. (1983) Control of gap junction formation in early mouse embryos. Devl. Biol. 98: 155–164.

    Article  CAS  Google Scholar 

  • Petzoldt, U., Hoppe, P.C., and Illmensee, K. (1980) Protein synthesis in enucleated fertilized and unfertilized mouse eggs. Wilhelm Roux’ Arch. Devl. Biol. 189: 215–219.

    Article  Google Scholar 

  • Rao, P.N., and Johnson, R.T. (1974) Regulation of cell cycle in hybrid cells. Cold Spring Harbor Conf. Cell Proliferation 1: 785–800.

    CAS  Google Scholar 

  • Sawicki, W., Abramczuk, J., and Blaton, O. (1978) DNA synthesis in the second and third cell cycles of the mouse preimplantation development Exp. Cell Res. 112: 199–205.

    Article  PubMed  CAS  Google Scholar 

  • Smith, L.C., Wilmut, L, and West, J.D. (1990) Control of first cleavage in single-ceil reconstituted mouse embryos. J. Reprod. Fert. 88: 655–663.

    Article  Google Scholar 

  • Telford, N.A., Watson, A.J., and Schultz, G.A. (1990) Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol. Reprod. Dev. 26: 90–100.

    Article  PubMed  CAS  Google Scholar 

  • Whittingham, D.C. (1971) Culture of mouse ova. J. Reprod. Fert. 14: 7–21.

    CAS  Google Scholar 

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© 1993 Springer Science+Business Media New York

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Wojcik, C., Mystkowska, E.T., Sawicki, W., Komar, A. (1993). Modulation of Nucleus Formation in Mouse Oocytes Fused with Early and Late G2 Blastomeres. In: Bach, P.H., Reynolds, C.H., Clark, J.M., Mottley, J., Poole, P.L. (eds) Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2828-9_25

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  • DOI: https://doi.org/10.1007/978-1-4615-2828-9_25

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6223-4

  • Online ISBN: 978-1-4615-2828-9

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