Skip to main content

Esterification in Micro Aqueous Organic Phase

  • Chapter
Developments in Food Engineering

Abstract

Esterification of oleic acid with lauryl alcohol, i.e. lauryl oleate, with lipase from Candida cylindracea, in micro aqueous phase was demonstrated. Di-2-ethlyehexyl sodium sulfosuccinate (AOT) was used as amphiphilic molecules in organic solvent. The initial reaction rate changed with the change in the molar ratio of water to amphiphilic molecules W. The maximum rate of esterification was obtained at W=12. The reaction kinetic parameters were determined quantitatively according to Michaelis-Menten analysis based on the sequential mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hayes, D.G., and Gulari, E. Esterification reaction of lipase in reverse micelles. Biotechnology and Bioenzineerinz., 1990, 35, 793–801.

    Article  CAS  Google Scholar 

  2. Rao, A.M., Murray, M.A., and John, VT. Characteristics of lipase catalysis during ester synthesis in reversed micellar system. Biocatalysis, 1991, 4, 253–64.

    Article  CAS  Google Scholar 

  3. Walde, P., Wao, Q., Bru, R., Luisi, P.L., and Kuboi, R. pH artifacts in reverse micellar enzymology: a warning. Pure & Awl. Chem., 1992, 64, 1771–75.

    CAS  Google Scholar 

  4. Chen, J., and Pai, H. Hydrolysis of milk fat with lipase in reversed micelles., Journal of Food Science, 1991, 56, 234–37.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Inoue, M., Imai, M., Shimizu, M. (1994). Esterification in Micro Aqueous Organic Phase. In: Yano, T., Matsuno, R., Nakamura, K. (eds) Developments in Food Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2674-2_157

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-2674-2_157

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6149-7

  • Online ISBN: 978-1-4615-2674-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics