Bacteriocins Produced by Carnobacterium Species

  • Michael E. Stiles

Abstract

The genus Carnobacterium was proposed by Collins et al. (1987) to include the atypical Lactobacillus -type bacteria isolated as part of the dominant ‘lactic’ microflora of chill-stored vacuum-packaged meats. Although the preservative effect of modified atmosphere packaging with elevated levels of carbon dioxide or vacuum packaging of meats was known by the 1930s, it was only in the 1960s that packaging technology was developed that allowed the meat industry to exploit this preservative effect to the full. These forms of anaerobic packaging caused public health concern for safety of meats packaged in this way, but studies on vacuum-packed sliced luncheon meats demonstrated that the lactic microflora that develops on these products provides protection against growth of mesophilic pathogenic bacteria (Stiles & Ng, 1979). Since the 1970s, the use of modified-atmosphere packaging and vacuum packaging has expanded dramatically and Carnobacterium has been shown to be an important genus growing on chill-stored meats (McMullen & Stiles, 1993).

Keywords

Fermentation Nitrite Trypsin Catalase Isopropanol 

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References

  1. Ahn, C. & Stiles, M. E. (1990a).Antibacterial activity of lactic acid bacteria isolated from vacuum-packaged meats. J. Appl. Bacteriol., 69, 302–310.CrossRefGoogle Scholar
  2. Ahn, C. & Stiles, M. E. (1990b).Plasmid-associated bacteriocin production by a strain of Carnobacterium piscicola from meat. Appl. Environ. Microbiol., 56, 2503–2510.Google Scholar
  3. Ahn, C. & Stiles, M. E. (1992).Mobilization and expression of bacteriocin plasmids from Carnobacterium piscicola isolated from meat. J. Appl. Bacteriol., 73, 217–228.CrossRefGoogle Scholar
  4. Ahn, C., Collins-Thompson, D., Duncan, C. & Stiles, M. E. (1992).Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum caTC2R. Plasmid, 27, 169–176.CrossRefGoogle Scholar
  5. Collins, M. D., Farrow, J. A. E., Phillips, B. A., Ferusu, S. & Jones, D. (1987).Classification of Lactobacillus divergens, Lactobacillus piscicola, and some catalase-negative, asporogenous, rod-shaped bacteria from poultry in a new genus, Carnobacterium. Int. J. Syst. Bacteriol., 37, 310–316.CrossRefGoogle Scholar
  6. Hastings, J. W., Sailer, M., Johnson, K., Roy, K. L., Vederas, J. C. & Stiles, M. E. (1991).Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J. Bacteriol., 173, 7491–7500.Google Scholar
  7. Hiu, S. R., Holt, R. A., Sriranganathan, N., Scidler, R. J. & Fryer, F. L. (1984).Lactobacillus piscicola, a new species from salmonid fish. Int. J. Syst. Bacteriol., 34, 393–400.CrossRefGoogle Scholar
  8. Holzapfel, W. H. & Gerber, E. S. (1983).Lactobacillus divergens sp. nov., a new heterofermentative Lactobacillus species producing L(+)-lactate. Syst. Appl. Microbiol., 4, 522–534.CrossRefGoogle Scholar
  9. Jewell, B. & Collins-Thompson, D. L. (1989).Characterization of chloramphenicol resistance in Lactobacillus plantarum caTC2R. Curr. Microbiol., 19, 343–346.CrossRefGoogle Scholar
  10. Lewus, C. B., Kaiser, A. & Montville, T. J. (1991).Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl. Environ. Microbiol., 57, 1683–1688.Google Scholar
  11. McMullen, L. M. & Stiles, M. E. (1993).Microbial ecology of fresh pork stored under modified atmosphere at-1, 4.4 and 10°C. Int. J. Food Microbiol., 18, 1–14.CrossRefGoogle Scholar
  12. Quadri, L. E. N., Sailer, M., Roy, K. L., Vederas, J. C. & Stiles, M. E. (1992). Characterization and genetic determinants of bacteriocins produced by Carnobacterium piscicola LV17B. American Society for Microbiology, Annual Meeting, New Orleans.Google Scholar
  13. Schillinger, U. & Holzapfel, W. H. (1990).Antibacterial activity of carnobacteria. Food Microbiol., 7, 305–310.CrossRefGoogle Scholar
  14. Schillinger, U., Stiles, M. E. & Holzapfel, W. H. (1993). Bacteriocin production by Carnobacterium piscicola LV61. Int. J. Food Microbiol. (submitted for publication).Google Scholar
  15. Shaw, B. G. & Harding, C. D. (1985).Atypical lactobacilli from vacuum-packaged meats: Comparison by DNA hybridization, cell composition and biochemical tests with a description of Lactobacillus carnis sp. nov. Syst. Appl. Microbiol., 6, 291–297.CrossRefGoogle Scholar
  16. Simons, G. & De Vos, W. M. (1987). Gene expression in lactic streptococci. In Proceedings of the 4th European Congress on Biotechnology, ed. O. M. Neijssel, R. R. van der Meer & K. Ch. A. M. Luyben. Vol. 1. Elsevier, Amsterdam, pp. 455–460.Google Scholar
  17. Stiles, M. E. & Ng, L.-K. (1979).Fate of pathogens inoculated onto vacuum-packaged sliced hams to simulate contamination during packaging. J. Food Prot., 42, 464–469.Google Scholar
  18. Stoffels, G., Nes, I. F. & Gudmundsdottir, A. (1992a).Isolation and properties of a bacteriocin-producing Carnobacterium piscicola isolated from fish. J. Appl. Bacteriol., 73, 309–316.CrossRefGoogle Scholar
  19. Stoffels, G., Nissen-Meyer, J., Gudmundsdottir, A., Sletten, K., Holo, H. & Nes, L. F. (1992b).Purification and characterization of a new bacteriocin isolated from a Carnobacterium sp. Appl Environ. Microbiol., 58, 1417–1422.Google Scholar
  20. Thornley, M. J. (1957).Observations on the microflora of minced chicken meat irradiated with 4 MeV cathode rays. J. Appl. Bacteriol., 20, 286–298.CrossRefGoogle Scholar
  21. Van Belkum, M. J., Hayema, B. J., Jeeninga, R. E., Kok, J. & Venema, G. (1991).Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ. Microbiol., 57, 492–498.Google Scholar
  22. Worobo, R. W., Henkel, T., Roy, K. L., Vederas, J. C. & Stiles, M. E. (1992). Characterization and genetic determinants of carnobacteriocin isolated from Carnobacterium piscicola LV17A. American Society for Microbiology, Annual Meeting, New Orleans.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Michael E. Stiles
    • 1
  1. 1.Department of Food ScienceUniversity of AlbertaEdmontonCanada

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