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Role of gene amplification in drug resistance

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Book cover Anticancer Drug Resistance

Part of the book series: Cancer Treatment and Research ((CTAR,volume 73))

Abstract

The drug-resistant phenotype is prevalent in many human cancers. In particular, multidrug resistance, in which cells are simultaneously resistant to several classes of anticancer agents, poses a major obstacle in the treatment of cancer. One strategy to elucidate drug resistance mechanisms operative in cancer cells is to independently derive drug resistant cell lines in vitro that mimic the drug resistance patterns observed in vivo. Drug-resistant mammalian cells selected in vitro may have highly amplified genomic sequences that encode large amounts of target proteins or large amounts of the detoxifying systems for a variety of cytotoxic agents [1,2]. These drug-resistant cell lines are particularly useful for the genetic, biochemical, and molecular analysis of drug resistance mechanisms because they provide excellent starting materials for the cloning of the respective drug resistance genes.

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Schoenlein, P.V. (1994). Role of gene amplification in drug resistance. In: Goldstein, L.J., Ozols, R.F. (eds) Anticancer Drug Resistance. Cancer Treatment and Research, vol 73. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2632-2_9

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