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Signal transduction through the cAMP-dependent protein kinase

  • Judy L. Meinkoth
  • Arthur S. Alberts
  • Wei Went
  • Dominic Fantozzi
  • Susan S. Taylor
  • Masatoshi Hagiwara
  • Marc Montminy
  • James R. Feramisco
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 11)

Abstract

Temporal cellular events responsible for hormonal activation of responses mediated by the cAMP-dependent protein kinase (PKA) have been studied in living cells. By selectively perturbing molecular function of Gs, the catalytic subunit of PKA (C), or the nuclear factor CREB, in cells through microinjection of inhibitory agents specific for these mole cules or activated forms of these molecules, we have obtained evidence for a requirement for the function of each of these molecules in the hormonal stimulation of cAMP-regulated genes. Moreover, by introducing fluorescently labeled PKA subunits into these cells as molecular tracers, or by immunofluorescence of C subunit, we have observed biological translocation of C subunit from the cytoplasm to the nucleus during transcriptional activation and a quenching of this by the inhibitor molecule, PKI. The implications of these cellular and molecular events in the signal transduction of hormonal responses are discussed. (Mol Cell Biochem 127/128: 179–186, 1993)

Key words

protein kinases signal transduction transcription activity phosphorylation microinjection 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Judy L. Meinkoth
    • 1
    • 4
  • Arthur S. Alberts
    • 2
  • Wei Went
    • 3
  • Dominic Fantozzi
    • 3
  • Susan S. Taylor
    • 3
    • 4
  • Masatoshi Hagiwara
    • 5
  • Marc Montminy
    • 5
  • James R. Feramisco
    • 1
    • 2
    • 4
  1. 1.Departments of MedicineUniversity of California at San DiegoLa JollaUSA
  2. 2.Departments of PharmacologyUniversity of California at San DiegoLa JollaUSA
  3. 3.Departments of ChemistryUniversity of California at San DiegoLa JollaUSA
  4. 4.Departments of Cancer CenterUniversity of California at San DiegoLa JollaUSA
  5. 5.The Clayton Foundation Laboratories for Peptide BiologyThe Salk InstituteLa JollaUSA

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