Elevated Ratio between Deoxycytidine Kinase and Thymidine Kinase 2 in CLL Lymphocytes Compared to Control Cells

  • Svend Erik Nielsen
  • Birgitte Munch-Petersen
  • Johannes Mejer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 370)

Abstract

Earlier studies comparing the phosporylation of Cytosine arabinoside (Ara-C) with deoxycytidine (dcyd) in leucocytes from patients with acute myeloid leukaemia (AML), chronic myeloid leukaemia (CML) and chronic lymphatic leukaemia (CLL) showed that the phosphorylation of ara-C was twice as high in the malignant cells than in the controls while there was no differences in the deamination of the two substrates (1, 2). An obvious explanation for the results was hard to give because purification of the involved enzymes was not carried out at time of the findings. Now both purification and substrate specificity of deoxycytidine kinase and thymidine kinase 2 has been done (3, 4).

Keywords

Catalysis Streptomycin Pyrimidine Thymidine Purine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mejer, J., Enzymatic studies on possible improvement of cytosine arabinoside treatment. Scand. J. Lab. Invest. 42, p. 401 (1982).CrossRefGoogle Scholar
  2. 2.
    Mejer, J., and Nygaard P. Cytosine arabinoside phosphorylation and deaminationin acute myeloblastic leukaemia cells. Leukaemia Research Vol. 2 No. 2 p. 127 (1977).CrossRefGoogle Scholar
  3. 3.
    Munch-Petersen, B., Cloos, L, Tyrsted, G., and Eriksson, S., Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosi-des. The Journal of Biological Chemistry, vol. 266, no. 14, p. 9032 (1990).Google Scholar
  4. 4.
    Eriksson, E., Kierdaszuk, B., Munch-Petersen, B., Öberg, B, and Johansson, N.G., Comparison of the substrate specificities of human thymidine kinase 1 and 2 and deoxycytidine kinase toward antiviral and cytostatic nucleoside analogs. Biochemical and biophysical research communications, vol. 176, no. 2, p. 586 (1991).PubMedCrossRefGoogle Scholar
  5. 5.
    Plunkett, W., Huang, P, Gandhi, V. Metabolism and action of Fludarabine Phosphate. Seminars in Oncology, vol 17, No 5, Suppl 8, p 3 (1990).PubMedGoogle Scholar
  6. 6.
    Gandhi, V., plunkett, W. Modulatory Activity of 2’, 2’-diflourodeoxycytidine on the phosphorylation and cytotoxicity of Arabinosyl Nucleosides. Cancer Research 50, p. 3675 (1990).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Svend Erik Nielsen
    • 1
  • Birgitte Munch-Petersen
    • 1
  • Johannes Mejer
    • 2
  1. 1.Department of Haematology and OncologyRoskilde County HospitalRoskildeDenmark
  2. 2.Institute of Life Sciences and ChemistryRoskilde University CentreRoskildeDenmark

Personalised recommendations