Elevated Ratio between Deoxycytidine Kinase and Thymidine Kinase 2 in CLL Lymphocytes Compared to Control Cells
Earlier studies comparing the phosporylation of Cytosine arabinoside (Ara-C) with deoxycytidine (dcyd) in leucocytes from patients with acute myeloid leukaemia (AML), chronic myeloid leukaemia (CML) and chronic lymphatic leukaemia (CLL) showed that the phosphorylation of ara-C was twice as high in the malignant cells than in the controls while there was no differences in the deamination of the two substrates (1, 2). An obvious explanation for the results was hard to give because purification of the involved enzymes was not carried out at time of the findings. Now both purification and substrate specificity of deoxycytidine kinase and thymidine kinase 2 has been done (3, 4).
KeywordsCatalysis Streptomycin Pyrimidine Thymidine Purine
Unable to display preview. Download preview PDF.
- 3.Munch-Petersen, B., Cloos, L, Tyrsted, G., and Eriksson, S., Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosi-des. The Journal of Biological Chemistry, vol. 266, no. 14, p. 9032 (1990).Google Scholar
- 4.Eriksson, E., Kierdaszuk, B., Munch-Petersen, B., Öberg, B, and Johansson, N.G., Comparison of the substrate specificities of human thymidine kinase 1 and 2 and deoxycytidine kinase toward antiviral and cytostatic nucleoside analogs. Biochemical and biophysical research communications, vol. 176, no. 2, p. 586 (1991).PubMedCrossRefGoogle Scholar