Abstract
2’-Deoxyuridine-5’-triphosphate-nucleotidohydrolase (dUTPase E.C. 3.6.1.23) serves two major functions in nucleotide metabolism, namely the production of dUMP, the immediate precursor of thymidine nucleotides, and the control of the intracellular dUTP level, thus minimising incorporation of uracil into DNA.
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References
Beck, W.R., Wright, G.E., Nusbaum, N.J., Chang, J.D., and Isselbacher, E.M., 1986, Enhancement of methotrexate cytotoxicity by uracil analogues that inhibit deoxyuridine triphosphate nucleotidohydrolase (dUTPase) activity, Adv. Exp. Med. Biol. 195B: 97.
Hochhauser, S.J. and Weiss, B., 1978, Escherichia coli mutants deficient in deoxyuridine triphosphatase, J. Bacteriol. 134: 157.
Hoffmann, I., Widström, J., Zeppezauer, M., and Nyman, P.O., 1987, Overproduction and large scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli, Eur. J. Biochem. 164: 45.
Kornberg, A., 1980, “DNA replication”, W.H Freeman & Co., San Francisco, USA.
Ludwig, J. and Eckstein, F., 1989, Rapid and efficient synthesis of nucleoside 5’-O-(l-thiotriphosphates), 5’-triphosphates and 2’,3’-cyclophosphorothioates using 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one, J. Org. Chem. 54: 631.
McIntosh, M., Ager, D.D., Gadsden, M.H., and Haynes, R.H., 1992, Human dUTP pyrophatase: cDNA sequence and potential biological importance of the enzyme, Proc. Nad. Acad. Sci. USA 89: 8020.
Mosman T., 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays, J. Immunol. Meth. 65: 55.
Tye, B.K., Nyman, P.O., Lehman, I.R., Hochhauser, S., and Weiss, B., 1977, Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA, Proc. Natl. Acad. Sci. USA 74: 154.
Zeppezauer, M., Zalud, P., Wachs, W.O., and Nyman, P.O., 1993, “Membrangängiger Wirkstoff zur Störung der DNA-Biosynthese”, German Patent Application P 4341 161.4; Dec 2, 1993.
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Zalud, P., Wachs, W.O., Nyman, P.O., Zeppezauer, M.M. (1995). Inhibition of the Proliferation of Human Cancer Cells In-Vitro by Substrate-Analogous Inhibitors of dUTPase. In: Sahota, A., Taylor, M.W. (eds) Purine and Pyrimidine Metabolism in Man VIII. Advances in Experimental Medicine and Biology, vol 370. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2584-4_31
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DOI: https://doi.org/10.1007/978-1-4615-2584-4_31
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