Advertisement

The Effect of Partial Hepatectomy on Blood Purine Levels in Rats and Patients

  • Makoto Usami
  • Koji Furuchi
  • Hiroshi Kasahara
  • Seiji Haji
  • George Kotani
  • Atsunori Iso
  • Kai Sun
  • Enmei Sou
  • Jyang-Hua Zheng
  • Kazuya Sakata
  • Kyosuke Ohta
  • Taichi Kanamaru
  • Yoichi Saitoh
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 370)

Abstract

Purine metabolism is a key metabolic process after partial hepatectomy in the synthesis of DNA and RNA, and in the energy metabolism for hepatic regeneration1. However it is not evaluated in clinical situations due to the difficulty in measuring changes of purine levels in the liver itself. In order to get a basic understanding of purine metabolism in clinical hepatectomy cases, blood samples are used for analysis in this study. Also, the liver is the main nondietary source of purines in mammalian cells2 and changes in blood level may affect purine metabolism in various tissues.

Keywords

Xanthine Oxidase Cirrhotic Patient Partial Hepatectomy Purine Nucleoside Purine Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. Itakura, T. Maeda, M. Tsuchiya, and K. Yamashita, Increased rate of de novo purine synthesis and its mechanism in regenerating liver, Am J Physiol. 251:G585 (1986).PubMedGoogle Scholar
  2. 2.
    A.W. Murry, The biological significance of purine salvage, Ann Rev Biochem 40: 811 (1971).CrossRefGoogle Scholar
  3. 3.
    V. Stocchi, L. Cucchiarine, F. Canestrari, M. Piacentini, and G. Fornani, A very fast ion-pair reversed-phase HPLC method for the separation of the most significant nucleotides and their degradation products in human red blood cells, Anal Biochem. 167:181 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    T. Yamamoto, Y. Moriwaki, S. Takahashi, T. Hada, and K. Higashino, Separation of hypoxanthine and xanthine from pyrazinamide and its metabolites in plasma and urine by high-performance liquid chromatography, J. Chromatogr. 382:270 (1986).PubMedGoogle Scholar
  5. 5.
    P. Kabasakalia, S. Kalliney, and A. Westcott, Enzymatic blood glucose determination by colorimetry of N, N-diethylailine-4-aminoantipyrine, Clin Chem. 20:606 (1974).Google Scholar
  6. 6.
    M. Usami, K. Furuchi, M. Ogino, J. Kotani, A. Iso, K. Oh, I. Yasuda, E. Sou, T. Kanamaru, H. Kasahara, Y. Saitoh, H. Yokoyama, and T. Yumisashi, Changes in purine and pyrimidine metabolism after partial hepatectomy in rats (in Japanese), Jap J Surg Metab Nutr. 27:35 (1993).Google Scholar
  7. 7.
    T. Kurokawa, T. Nonami, K. Kuroe, M. Satake, A. Harada, A. Nakano, and H. Takagi, Nucleotide metabolism in remnant rat liver after major hepatic resection (in Japanese), J Jap Surg Soc. 9:994 (1990).Google Scholar
  8. 8.
    S.H. Giler, O. Sperling, S. Brosh, I. Urca, and A. De Vries, Serum xanthine oxidase in jaundice, Clin Chim Acta. 63:37 (1975).PubMedCrossRefGoogle Scholar
  9. 9.
    S. Ogoshi, M. Iwasa, T. Yonezawa, and T. Tamiya, Effect of nucleotide and nucleoside mixture on rats given total parenteral nutrition after 70% hepatectomy, J. Parenter Enterai Nutr. 9:339 (1985).CrossRefGoogle Scholar
  10. 10.
    H. Ohyanagi, S. Nishimatu, Y. Kanbara, M. Usami, and Y. Saitoh, Effect of nucleosides and a nucleotide on DNA and RNA syntheses by the salvage and de novo pathway in primary monolayer cultures of hepatocytes and hepatoma cells, J Parenter Enterai Nutr. 13:51 (1989).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Makoto Usami
    • 1
  • Koji Furuchi
    • 1
  • Hiroshi Kasahara
    • 1
  • Seiji Haji
    • 1
  • George Kotani
    • 1
  • Atsunori Iso
    • 1
  • Kai Sun
    • 1
  • Enmei Sou
    • 1
  • Jyang-Hua Zheng
    • 1
  • Kazuya Sakata
    • 1
  • Kyosuke Ohta
    • 1
  • Taichi Kanamaru
    • 1
  • Yoichi Saitoh
    • 1
  1. 1.First Department of SurgeryKobe University School of MedicineChuo-ku, Kobe, 650Japan

Personalised recommendations