Rapid Simultaneous Measurement of Nucleotides, Nucleosides and Bases in Tissues by Capillary Electrophoresis
Capillary electrophoresis (CE) is now one of the most efficient separation techniques. It has been rapidly developed since Jorgenson et al.1 used small diameter capillaries for the separation of ions in an electrical field. One of the greatest advantages of CE is the use of small sample amounts — from some few nl of sample to the cytosolic fluid of single cells2. These small sample volumes make it possible to solve a number of biological and clinical problems, where the amount of biological material is limited.
KeywordsUric Acid Capillary Electrophoresis Capillary Zone Electrophoresis Inosine Monophosphate Micellar Electrokinetic Capillary Chromatography
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- 4.M. Ng, T.F. Blaschke, A.A. Arias and R.N. Zare. Analysis of free intracellular nucleotides using high-performance capillary electrophoresis. And Chem, 64: 1682 (1992).Google Scholar
- 9.D. Perrett and G. Ross. Capillary electrophoresis for the analysis of cellular nucleotides. In Purine and Pyrimidine Metabolism in Man VII Part B, ed. G.B. Elion, R.A. Harkness and N. Zollner„ 1–5. New York. Plenum Press. (1991).Google Scholar
- 15.L. Hernandez, B.G. Hoebel and N.A. Guzman. Analysis of cyclic nucleotides by capillary electrophoresis using ultraviolet detection. In Analytical Biotechnology. Capillary Electrophoresis and Chromatography (ACS Sym Series No 434), ed. C. Horvath and J. Nikelly„ 50–59. Washington DC. American Chemical Society.(1990).CrossRefGoogle Scholar