Advertisement

Mutagenesis Studies of Conserved Residues in Mammalian Dihydroorotase

  • Barbara H. Zimmermann
  • Nancy M. Kemling
  • David R. Evans
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 370)

Abstract

Dihydroorotase (DHOase3) (L-5, 6-dihydroorotate amidohydrolase, EC 3.4.2.3) catalyzes the reversible cyclization of carbamyl aspartate to form dihydroorotate, the third step in de novo pyrimidine biosynthesis. In mammals, the activity is carried by the multifunctional protein CAD, which also carries the activities of the first two steps in the pathway, carbamyl phosphate synthetase (CPSase) and aspartate transcarbamylase (ATCase). The hamster CAD molecule is a hexamer consisting of six identical Polypeptides, each having a molecular weight of 242 kDa. Limited proteolysis and sequence analysis have shown that the DHOase activity is associated with a discrete domain of the protein (Kelly et al., 1986; Simmer et al., 1990).

Keywords

Limited Proteolysis Mutant D1512N Pyrimidine Biosynthesis Carbamyl Phosphate Reversible Cyclization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bradford, M. (1976) Analyt. Biochem. 72, 248–254.PubMedCrossRefGoogle Scholar
  2. Christopherson, R. I. & Jones, M. E. (1979) J. Biol. Chem. 254, 12506–12512.PubMedGoogle Scholar
  3. Christopherson, R. I. & Jones, M. E. (1980) J. Biol. Chem. 255, 3358–3370.PubMedGoogle Scholar
  4. Kelly, R. E., Mally, M. I., & Evans, D. R. (1986) J. Biol Chem. 261, 6073–6083.PubMedGoogle Scholar
  5. Kunkel, T. A. (1985) Proc. Nat. Acad. Sci. U.S.A. 82, 488–492.CrossRefGoogle Scholar
  6. Nowlan, S. F. & Kantrowitz, E. R. (1985) J. Biol Chem. 260, 14712–14716.PubMedGoogle Scholar
  7. Prescott, L. M., & Jones, M. E. (1969) Anal. Biochem. 32, 408–419.PubMedCrossRefGoogle Scholar
  8. Semple, K. S., & Silbert, D. F. (1975) J. Bacteriol. 121, 1036–1046.PubMedGoogle Scholar
  9. Simmer, J. P., Kelly, R. E., Scully, J. L., Grayson, D. R., Rinker, A. G., Jr., Zimmermann, B. H., Kim, H., & Evans, D. R. (1990) Proc. Nat. Acad. Sci. U.S.A. 87, 174–178.CrossRefGoogle Scholar
  10. Vallee, B. L. & Auld, D. S. (1990) Biochemistry 29, 5647–5659.PubMedCrossRefGoogle Scholar
  11. Zimmermann, B. H. & Evans, D. R. (1993) Biochemistry 32, 21519–21527.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Barbara H. Zimmermann
    • 1
  • Nancy M. Kemling
    • 2
  • David R. Evans
    • 2
  1. 1.Department of BiochemistryUniversity of Puerto Rico Medical Sciences CampusSan JuanUSA
  2. 2.Department of BiochemistryWayne State UniversityDetroitUSA

Personalised recommendations