Observed Resistance to Pyrimidine Analogs and Sensitivity to Uracil in Drosophila is attributed to Deregulation of Pyrimidine Metabolism
Pyrimidine nucleotides play a central role in cellular metabolism and regulation. In most organisms two pathways provide pyrimidines: the de novo biosynthetic pathway and the salvage pathway. Drosophila melanogaster, the fruit fly, is an ideal organism for study of the genetic basis and regulatory mechanisms of various metabolic pathways. De novo pyrimidine biosynthesis in the fruit fly is a six-step pathway, which is catalyzed by enzymes encoded by three separate genes (Freund and Jarry, 1987; Rawls et al., 1993; Eisenberg et al., 1993). The gene rudimentary (r) is Drosophila’s equivalent of the mammalian gene for CAD (Freund and Jarry, 1987). De novo pyrimidine biosynthesis is important for the proper development of flies. However, the salvage pathway can suffice when the external supply of pyrimidines is very high (Falk and Nash, 1974).
KeywordsMutant Allele Wild Type Allele Catabolic Pathway Salvage Pathway Pyrimidine Nucleotide
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