Do Bacterial Membranes Contribute to Calcium Oxalate (Caox) Crystal Deposition?
Clinical studies demonstrate the presence of bacteria in as many as one third of calcium oxalate/phosphate stones1. A role for such organisms has not been previously recognized. Bacteria may develop intracellular calcium phosphate crystals (hydroxyapatite) in unique clinical situations (e.g. dental tartar) and when inoculated into calcium phosphate enriched growth media in vitro 2. Previous work in our laboratory suggests that bacteria may form intracellular electron dense deposits containing calcium when inoculated in human urine3. Such bacteria demonstrate x-ray powder diffraction patterns consistent with hydroxyapatite3, 4. Additional in vitro studies suggest that bacteria may form microcolonies on tissue surfaces in urines that demonstrate increased levels of calcium5. The following study was designed to test the hypothesis that bacteria or bacterial membranes (mineralized and non-mineralized) may form a platform for calcium oxalate crystal deposition.
KeywordsCalcium Oxalate Bacterial Membrane Crystal Deposition Calcium Oxalate Crystal Calcium Chloride Solution
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