The Use of the Multiblock in Diagnostic Immunohistochemistry

Abstract

In immunohistochemistry, the availability of a steadily increasing number of antibodies allows for a more comprehensive characterization of tissue components. Often, very large numbers of slides are easily generated when panels of antibodies are applied to a series of cases, as in clinical research.

To save time and space and prevent generation of large numbers of slides, we have developed the multiblock technology. The multiblock is a conventional size paraffin block, composed of up to 24 different tissue samples. These samples are punched from preexisting paraffin blocks and reembedded together.

The multiblock is then routinely sectioned and immunostained. The multiblock method facilitates the immunostaining and quicker evaluation of samples. As a result of the ability to decrease the number of immunostaining rounds, certain variations are eliminated. Multiblocks can also be used for routine laboratory tests on available antibodies and quality control.