Small-Angle Neutron Scattering Studies of Proteoglycan from Shark Fin Cartilage
The gross structural properties, size and shape of the proteoglycan complex from shark fin cartilage in native state were determined by the small-angle neutron scattering (SANS) method in different D2O/H2O ratio. A contrast matching point of the complex was estimated to be 53% D2O/H2O. The dependence of radius of gyration on the solvent contrast was positive, indicating that the mucopolysaccharide is on the surface of the core protein. The proteoglycan complex in native state was approximated as the two uniform concentric ellipsoidal shell model having the axial ratio of about 3 in SANS method.
KeywordsChondroitin Sulfate Axial Ratio Keratan Sulfate Scatter Length Density Japan Atomic Energy Research Institute
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