The Relationship of Radiation Sensitivity and Microenvironment of Human Tumor Cells in Multicellular Spheroid Tissue Culture

  • A. Görlach
  • H. Acker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 345)

Abstract

Despite many publications describing a correlation between radioresistance and radiobiological hypoxia (Adams, 1990; Hill and Pallavicini,1984; Rockwell and Moulder, 1990) the importance of this phenomenon is still under discussion as well as the mechanisms relevant for the outcome to radiotherapy (Deacon et al.,1984; Steel and Peacock,1989).

Keywords

Lactate Adenocarcinoma Agarose Penicillin Pyruvate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams, G.E., 1990, The clinical relevance of tumour hypoxia, Eur. J. Cancer, 26:420–421.PubMedCrossRefGoogle Scholar
  2. Bergmeyer, H.U., and Bernt, E., 1974, Lactate dehydrogenase, in: “Methods of Enzymatic Analysis,” H.U. Bergmeyer, ed., Academic Press, New York-London.Google Scholar
  3. Carlsson, J., and Acker, H., 1988, Relations between pH, oxygen partial pressure and growth in cultured cell spheroids, Int. J. Canc. 42:715–720.CrossRefGoogle Scholar
  4. Deacon, J., Peckham, M.J., and Steel, G.G., 1984, The radioresponsiveness of human tumours and the initial slope of the cell survival curve, Radioth. Oncol. 2:317–323.CrossRefGoogle Scholar
  5. Freitas, I., and Baronzio, G.F., 1991, Tumor hypoxia, reoxygenation and oxygenation strategies: possible role in photodynamic therapy, J. Photochenn. Photobiol. B: Biol. 11:3–30.CrossRefGoogle Scholar
  6. Grossmann, U., 1984, Profiles of oxygen partial pressure and oxygen consumption inside multicellular spheroids, Rec. Res. Cancer Res. 95:150–161.CrossRefGoogle Scholar
  7. Grote, J., Süsskind, R., and Vaupel, P., 1977, Oxygen diffusivity in tumor tissue (DS-Carcinosarcoma) under temperature conditions within the range of 20–40°C, Pflügers Arch. 372:37–42.PubMedCrossRefGoogle Scholar
  8. Haveman, J., 1980, The influence of pH on the survival after X-irradiation of cultured malignant cells. Effects of carbonylcyanide-3-chlorophenylhydrazone, Int. J. Rad. Biol. 37:201–205.CrossRefGoogle Scholar
  9. Hill, R.P., and Pallavicini, M.G., 1983, Hypoxia and the radiation response of tumors, Adv. Exp. Med. Biol. 159:17–35.PubMedCrossRefGoogle Scholar
  10. Holtermann, G., and Acker, H., 1992, Easy producible amperometric and voltametric glass microsensors with tip-diameters between 0.3 and 3µm, in: “Biosensors: Fundamentals, Technologies and Applications,” F. Scheller, R.D. Schmid, eds., VCH, Weinheim, FRG.Google Scholar
  11. Murray, R.K., 1987, Biochemical properties of cancer cells, in: “The Basic Science of Oncology,” I.F. Tannock, R.P. Hill, eds., Pergamon Press, New York.Google Scholar
  12. Nylen, T., Acker, H., Bölling, B., Holtermann, G., and Carlsson J., 1989, Influence of ionizing radiation on oxygen profiles in different types of multicellular spheroids, Rad. Res. 120:213–226.CrossRefGoogle Scholar
  13. Rockwell, S., and Moulder, J.E., 1990, Hypoxic fractions of human tumors xenografted into mice, Int. J. Rad. Onc. 19:197–202.CrossRefGoogle Scholar
  14. Röttinger E.M., and Mendonca, M., 1982, Radioresistance secondary to low pH in human glial cells and Chinese hamster ovary cells, Int. J. Rad. Onc. 8:1309–1314.CrossRefGoogle Scholar
  15. Schwachöfer, J.H.M., Crooijmans, R.P.M.A., Hoogenhout, J., Jerusalem, C.R., Kal, H.B., and Theeuwes, A.G.M., 1990, Radiosensitivity of human melanoma spheroids influenced by growth rate, Int. J. Rad. One. 19:1191–1197.CrossRefGoogle Scholar
  16. Schwachöfer, J.H.M., Acker, H., Crooijmans, R.P.M.A., van Gasteren, R.P.M.A., Holtermann, G., Hoogenhout, J., Jerusalem, C.R., and Kal, H.B., 1991, Oxygen tension in two human tumor cell lines grown and irradiated as multicellular spheroids, Anticancer Res. 11:1365–1368.Google Scholar
  17. Steel, G.G., and Peacock, J.H., 1989, Why are some tumors more radiosensitive than others? Radioth. Oncol. 15:63–72.CrossRefGoogle Scholar
  18. Sutherland, R.M., 1988, Cell and environment interactions in tumor microregions: The multicell spheroid model, Science 240:177–184.PubMedCrossRefGoogle Scholar
  19. Wike-Hooley, J.L., Haveman, J., and Reinhold, H.S., 1984, The relevance of tumor pH to the treatment of malignant disease, Radiother. Oncol. 2:343–366PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • A. Görlach
    • 1
  • H. Acker
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmund 1Germany

Personalised recommendations