Human Cytotoxic T Cell Epitopes in HPV 11: Relationships between Allele-Specific Motifs, HLA Binding, and Stimulation in Vitro
The identification of cytotoxic T lymphocyte (CTL) epitopes in HPV may offer a route to peptide vaccine design. However, the screening of suitable peptides from a virus usually requires CTL that have been elicited during natural. infection. The difficulty in obtaining viable HPV (used to infect stimulator cells for in vitro propagation of naturally-occurring CTL) has complicated the issue. One approach to overcome this is to prime CTL in vitro with HPV antigen processed endogenously and presented by dendritic cells (I. Tarpey, S. Stacey, J. Hickling, H. Birley, A. Renton, A. McIndoe and H. Davies; Immunol, in press). This allows the peptides of endogenous processing to be presented to T cells which may mimic the events that occur in vivo. There are other approaches to locating epitopes that are independent of pre-existing CTL. These include screening of candidate peptides for binding to HLA molecules, or to predict epitopes on the basis of possession HLA allele-specific motifs.
KeywordsVaccinia Virus Recombinant Chromium Release Assay Influenza Virus Matrix Protein
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