Advertisement

Human Cytotoxic T Cell Epitopes in HPV 11: Relationships between Allele-Specific Motifs, HLA Binding, and Stimulation in Vitro

  • Huw Davies
  • Ian Tarpey
  • Simon Stacey
  • Julian Hickling
  • Jennifer Bartholomew
  • Humphrey Birley
  • Adrian Renton
  • Angus McIndoe

Abstract

The identification of cytotoxic T lymphocyte (CTL) epitopes in HPV may offer a route to peptide vaccine design. However, the screening of suitable peptides from a virus usually requires CTL that have been elicited during natural. infection. The difficulty in obtaining viable HPV (used to infect stimulator cells for in vitro propagation of naturally-occurring CTL) has complicated the issue. One approach to overcome this is to prime CTL in vitro with HPV antigen processed endogenously and presented by dendritic cells (I. Tarpey, S. Stacey, J. Hickling, H. Birley, A. Renton, A. McIndoe and H. Davies; Immunol, in press). This allows the peptides of endogenous processing to be presented to T cells which may mimic the events that occur in vivo. There are other approaches to locating epitopes that are independent of pre-existing CTL. These include screening of candidate peptides for binding to HLA molecules, or to predict epitopes on the basis of possession HLA allele-specific motifs.

Keywords

Vaccinia Virus Recombinant Chromium Release Assay Influenza Virus Matrix Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    K. Falk, O. Rötzschke, S. Stevanovic, G. Jung, and H-G. Rammensee. Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules. Nature. 351:290 (1991).PubMedCrossRefGoogle Scholar
  2. 2.
    M. DiBrino, Parker, K. C., Shiloach, J., Knierman, M., Lukszo, J., Turner, R. V., Biddison, W. E. and Coligan, J. E. Endogenous peptides bound to HLA-A3 possess a specific combination of anchor residues that permit identification of potential. antigenic epitopes. Proc Natl Acad Sci USA. 90,1508–1512 (1993).PubMedCrossRefGoogle Scholar
  3. 3.
    V. Cerundolo, J. Alexander, K. Anderson, C. Lamb, P. Cresswell, A. McMichael, F. Gotch, and A. Townsend. Presentation of viral. antigen controlled by a gene in the major histocompatibility complex. Nature. 345:449 (1990).PubMedCrossRefGoogle Scholar
  4. 4.
    S. E Macatonia, S. Paterson, and S. C. Knight. Primary proliferative and cytotoxic T cell responses to HIV induced in vitro by human dendritic cells. Immunol. 74:399 (1991).Google Scholar
  5. 5.
    F. Gotch, A. McMichael, and J. Rothbard Recognition of influenza A matrix protein by HLA A2-restricted cytotoxic T lymphocytes. Use of analogues to orientate the matrix peptide in the HLA A2 binding site. J Exp Med. 168:2045 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Huw Davies
    • 1
  • Ian Tarpey
    • 1
  • Simon Stacey
    • 2
  • Julian Hickling
    • 3
  • Jennifer Bartholomew
    • 2
  • Humphrey Birley
    • 4
  • Adrian Renton
    • 5
  • Angus McIndoe
    • 6
  1. 1.Division of Life SciencesKing’s CollegeLondonUK
  2. 2.CRC Paterson Institute for Cancer ResearchChristie Hospital NHS TrustManchesterUK
  3. 3.ICRF, Human Tumour Immunology GroupUniversity College and Middlesex School of MedicineLondonUK
  4. 4.Departments of Genitourinary MedicineSt. Mary’s HospitalLondonUK
  5. 5.Departments of Public HealthSt. Mary’s HospitalLondonUK
  6. 6.Departments of Obstetrics and GynaecologySt. Mary’s HospitalLondonUK

Personalised recommendations