Abstract
Major advances have occured in the structural analysis of mucins found in breast cancer following the isolation of cDNAs and the gene for MUC1 - which is the predominant mucin expressed in breast cancer1-4. In addition, other genes coding for mucins have been identified which are expressed in a variety of tissues, and are also found in breast tissue and to a lesser extent in breast cancer (particularly MUC2 and MUC3)5, 6 It was of interest that these genes were isolated using polyclonal antibodies and bacterial expression libraries, indicating that the active antibodies were detecting a non-glycosylated linear peptide, and this prediction was proven by the finding that many existing anti-MUC1 monoclonal antibodies react with synthetic peptides. The focus of this review will be to determine whether: a) monoclonal antibodies made against tumors react with peptides and what epitopes are detected (particularly for MUC1); b) if anti-pep tide antibodies can be made which react with cancers (MUC1,2, 3); c) second generation antibodies can be made to fusion proteins (MUC1); d) and what epitopes are detected with all of these antibodies; e) finally, what are the implications of these new advances in the diagnosis and treatment of breast cancer.
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Xing, PX., Apostolopoulos, V., Trapani, J., Prenzoska, J., McKenzie, I.F.C. (1994). Peptide Epitopes in Breast Cancer Mucins. In: Ceriani, R.L. (eds) Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment. Advances in Experimental Medicine and Biology, vol 353. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2443-4_2
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DOI: https://doi.org/10.1007/978-1-4615-2443-4_2
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