Advertisement

Genetic Transformation of Wheat

  • Indra K. Vasil
  • Vimla Vasil
  • Vibha Srivastava
  • Ana M. Castillo
  • Michael E. Fromm

Summary

Transgenic plants of two spring and one winter cultivar of wheat were produced by the direct introduction of DNA, either into long-term regenerable callus cultures, or into immature embryos and young calli. The presence of the herbicide resistant bar gene was demonstrated by phosphinothricin acetyltransferase activity, resistance to the herbicide basta, and DNA hybridization. The bar gene segregated as a dominant Mendelian trait in sexual progeny.

Keywords

Somatic Embryo Embryogenic Callus Immature Embryo Sexual Progeny Winter Cultivar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ozias-Akins, P. and Vasil, I.K. (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): evidence for somatic embryogenesis. Protoplasma 110, 95–105.CrossRefGoogle Scholar
  2. Redway, F.A., Vasil, V., Lu, D. and Vasil, I.K. (1990a) Identification of callus types for long term maintenance and regeneration from commercial cultivars of wheat Triticum aestivum L.). Theoret. Appl. Genet. 79, 609–617.CrossRefGoogle Scholar
  3. Redway, F.A., Vasil, V. and Vasil, I.K. (1990b) Characterization and regeneration of wheat (Triticum aestivum L.) from embryogenic cell suspension cultures. Pl. Cell Rep. 8, 714–717.CrossRefGoogle Scholar
  4. Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. (1988) Genetically transformed maize plants from protoplasts. Science 240, 204–207.PubMedCrossRefGoogle Scholar
  5. Vasil, I.K. (1993) Molecular genetic improvement of cereal and grass crops. IAPTC Newsletter (in press).Google Scholar
  6. Vasil, I.K. and Vasil, V. (1992) Advances in cereal protoplast research. Physiol. Plant. 85, 279–283.CrossRefGoogle Scholar
  7. Vasil, I.K. and Vasil, V. (1993) In vitro culture of cereals and grasses. In: Plant Cell and Tissue Culture, I.K. Vasil and T.A. Thorpe (eds.), Kluwer Academic Publishers, Dordrecht.Google Scholar
  8. Vasil, V, Brown, S.M., Re, D., Fromm, M.E. and Vasil, I.K. (1991) Stably transformed callus lines from micro-projectile bombardment of cell suspension cultures of wheat. Bio/Technology 9, 743–747.CrossRefGoogle Scholar
  9. Vasil, V, Castillo, A.M., Fromm, M.E. and Vasil, I.K. (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10, 667–674.CrossRefGoogle Scholar
  10. Vasil, V, Redway, F.A. and Vasil, I.K. (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technology 8, 429–433.CrossRefGoogle Scholar
  11. Vasil, V, Srivastava, V, Castillo, A.M., Fromm, M.E. and Vasil, I.K. (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11, 1553–1558.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Indra K. Vasil
    • 1
  • Vimla Vasil
    • 1
  • Vibha Srivastava
    • 1
  • Ana M. Castillo
    • 1
  • Michael E. Fromm
    • 2
  1. 1.Laboratory of Plant Cell and Molecular BiologyUniversity of FloridaGainesvilleUSA
  2. 2.Monsanto CompanySt. LouisUSA

Personalised recommendations