Genetic Transformation of Wheat
Transgenic plants of two spring and one winter cultivar of wheat were produced by the direct introduction of DNA, either into long-term regenerable callus cultures, or into immature embryos and young calli. The presence of the herbicide resistant bar gene was demonstrated by phosphinothricin acetyltransferase activity, resistance to the herbicide basta, and DNA hybridization. The bar gene segregated as a dominant Mendelian trait in sexual progeny.
KeywordsSomatic Embryo Embryogenic Callus Immature Embryo Sexual Progeny Winter Cultivar
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- Vasil, I.K. (1993) Molecular genetic improvement of cereal and grass crops. IAPTC Newsletter (in press).Google Scholar
- Vasil, I.K. and Vasil, V. (1993) In vitro culture of cereals and grasses. In: Plant Cell and Tissue Culture, I.K. Vasil and T.A. Thorpe (eds.), Kluwer Academic Publishers, Dordrecht.Google Scholar