Abstract
We used synthetic peptides duplicating the structures of a human λ light chain (Mcg), and a human T-cell receptor (Tcr) α and a Tcr β chain predicted from gene sequence to determine the presence and loci of activity of natural human autoantibodies directed against these antigen recognition molecules. We report that normal individuals and patients suffering from autoimmune diseases have antibodies directed against regions of λ light chains and Tcr ß chains corresponding to the first complementarity determining region and the third framework region of the variable domain and to constant region determinants. The levels of IgM natural antibodies particularly against the CDR1 peptides tend to be higher in RA patients than in normals or SLE patients. Although polyclonal IgG immunoglobulins from healthy individuals did not show detectable reactivity to Tcr α peptides, such reactivity was found in the IgM immunoglobulins of RA patients, thereby showing that Tcr α peptides can be autoantigenic in man. The levels of IgM autoantibodies to Vß CDR1 peptides tend to decrease with age. By contrast, there was a marked increase in IgG natural autoantibodies to certain CDR1 sequences with advancing age. We suggest that the natural antibodies to defined regions of immunoglobulins and T-cell receptors are part of a physiological network for the regulation of the immune response.
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Marchalonis, J.J., Kaymaz, H., Schluter, S.F., Yocum, D.E. (1994). Naturally Occurring Human Autoantibodies to Defined T-Cell Receptor and Light Chain Peptides. In: Atassi, M.Z. (eds) Immunobiology of Proteins and Peptides VII. Advances in Experimental Medicine and Biology, vol 347. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2427-4_14
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DOI: https://doi.org/10.1007/978-1-4615-2427-4_14
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