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The Cell Cycle pp 263-269 | Cite as

Cell Proliferation as a Biomarker of Age and Development

  • M. H. Lu
  • S. F. Ali
  • D. S. He
  • A. Turturro
  • R. W. Hart
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Changes in the proliferative capacity of cells can serve as a biomarker of development, age, and carcinogenesis. Perturbation of the cell cycle is considered a toxic endpoint. A paucity of data is available on the cell cycle as a function of age. in order to understand the etiology of ontogenesis and carcinogenesis, it is essential that background information be established on cell proliferation. The brain is known to have a very high level of proliferation during the early stages of development which decreases with age. The present study was designed to establish background information on cell proliferation, by cell cycle analysis, in the brain of normal male rats during development. Brain tissues of Sprague-Dawley rats (newborn, 21 days, 6 months, and one year of age) were dissected to obtain the cerebrum, cerebellum, and brain stem. Cellular proliferation activity was determined by flow cytometry. The percentage of S-phase cells was used to establish the proliferative index (PI). Our results indicate that there are differences in proliferation among the three brain regions in all age groups except for the 6-month old animals. in all cases, cell proliferation was highest in newborn rats. Proliferation activity in the cerebrum of newborn and 21-day old rats was equivalent (17%); after 21 days of age, proliferative activity decreases and maintains the same low rate (about 6.5%) until at least one year of age. The proliferation activity of cerebellum (21% at birth) decreases in animals at day 21 (7%) and the decrease continues until 6 months of age at which time it remains constant (5%) for at least one year. The proliferative activity of brain stem decreases from 16% to 5% immediately after birth and maintains the same low rate from day 21 to one year of age.

Keywords

Brain Stem Proliferative Activity Proliferative Index Cell Cycle Analysis External Granular Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • M. H. Lu
    • 1
  • S. F. Ali
    • 1
  • D. S. He
    • 1
  • A. Turturro
    • 1
  • R. W. Hart
    • 1
  1. 1.National Center for Toxicological ResearchFood and Drug AdministrationJeffersonUSA

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