A GTPase Cycle Coupled to the Cell Cycle

  • Elias Coutavas
  • Mindong Ren
  • Joel D. Oppenheim
  • Vijay Yajnik
  • Peter D’Eustachio
  • Mark G. Rush
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Ran/TC4, a Ras-related nuclear protein first identified in a human teratocarcinoma eDNA library, is postulated to function in conjunction with another protein, RCC1 (Regulator of Chromosomal Condensation-1), to prevent the initiation of mitosis before the completion of DNA synthesis. RCC1 is a chromatin-associated DNA-binding protein as well as a Ran/TC4-specific guanine nucleotide exchange factor. One model for the function of Ran/TC4 and RCC1 is that RCC1 monitors DNA synthesis and maintains Ran/TC4 in a GTP-bound form (Ran/TC4 •GTP) until the completion of DNA synthesis and that Ran/TC4 • GTP in turn inhibits the activation of MPF (Mitosis Promoting Factor). We are testing this model, in which a GTPase cycle is coupled to the cell cycle via MPF by ex-pressing mutant Ran/TC4 proteins in human cells, and by using a Ran/TC4 - [32P]GTP complex as a probe to isolate Ran-interacting proteins.


Nonpermissive Temperature Premature Chromosome Condensation HeLa Cell Extract GTPase Cycle tsBN2 Cell 
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Elias Coutavas
    • 1
  • Mindong Ren
    • 2
  • Joel D. Oppenheim
    • 3
  • Vijay Yajnik
    • 4
  • Peter D’Eustachio
    • 1
    • 5
  • Mark G. Rush
    • 1
    • 5
  1. 1.Department of BiochemistryNYU Medical CenterNew YorkUSA
  2. 2.Department of Cell BiologyNYU Medical CenterNew YorkUSA
  3. 3.Department of MicrobiologyNYU Medical CenterNew YorkUSA
  4. 4.Department of PharmacologyNYU Medical CenterNew YorkUSA
  5. 5.Kaplan Cancer CenterNYU Medical CenterNew YorkUSA

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