Rapid Measurement of Selected Tear Proteins in Health and Disease Using the Touch Tear Microassay System

  • Gary N. Foulks
  • Keith Baratz
  • Phil Ferrone
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 350)


Measurement of tear fluid immunoglobulins has been restricted to investigational studies due to the complexity of testing methods, yet many studies have demonstrated association of elevated tear IgE with allergic disease of the external eye1–7. The methods of testing have also been hampered by need for a relatively large volume of tear fluid and the attendant induced reflex tearing at the time of collection resulting in artefactually low values of IgE. The Touch Tear Microassay System was designed to adapt solid-phase ELISA testing with sensitive reflectometric determination of color density reaction during the measurement of protein levels in very small volume tear samples (2 µl). The present clinical study attempted to quantitate levels of IgE, IgG and C-reactive protein in normal patients and patients with external ocular signs and symptoms due to allergic and infectious disease using the Touch Tear Microassay System.


Allergic Conjunctivitis Bacterial Conjunctivitis Seasonal Allergic Conjunctivitis Vernal Keratoconjunctivitis Giant Papillary Conjunctivitis 
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Gary N. Foulks
    • 1
  • Keith Baratz
    • 1
  • Phil Ferrone
    • 1
  1. 1.Duke University Eye CenterDurhamUSA

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