Functional Aspects of Three Modified Nucleosides, Ψ, ms2io6A, and m1G, Present in the Anticodon Loop of tRNA

  • Tord G. Hagervall
  • Birgitta Esberg
  • Ji-nong Li
  • Thérèse M. F. Tuohy
  • John F. Atkins
  • James F. Curran
  • Glenn R. Björk

Abstract

Transfer RNAs contain many modified nucleosides, which are derivatives of the four normal nucleosides. At present more than 75 different modified nucleosides are characterised (Edmonds et al., 1991). The synthesis of the majority of the modified nucleosides is carried out on the preformed precursor tRNA except in two cases. Queuine and hypoxanthine are synthesised from guanine and adenine, respectively, and then incorporated into the tRNA through an exchange reaction. The synthesis of these 75 modified nucleosides is catalysed by enzymes, which are highly specific, not only for the nucleoside that they modify but also for the position of the target nucleoside in the tRNA. For example there are different enzymes catalysing the formation of Ψ in the anticodon stem and in the TΨC-loop (Singer et al., 1972). The importance of tRNA modification is reflected by the fact that about 1% of the genetic information in Escherichia coli and Salmonella typhimurium is devoted to the synthesis of tRNA modifying enzymes, which is 4-fold more than that used for the synthesis of their substrate, tRNA (Björk, 1992). This paper will discuss the function of three modified nucleosides present in the anticodon region. Mutants defective in their synthesis have been used to study their role in cell physiology and in the decoding process.

Keywords

Codon Cysteine Adenosine Proline Arginine 

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Tord G. Hagervall
    • 1
  • Birgitta Esberg
    • 1
  • Ji-nong Li
    • 1
  • Thérèse M. F. Tuohy
    • 2
  • John F. Atkins
    • 2
  • James F. Curran
    • 3
  • Glenn R. Björk
    • 1
  1. 1.Department of MicrobiologyUniversity of UmeåUmeåSweden
  2. 2.Howard Hughes Medical Institute and Department of Human GeneticsUniversity of UtahSalt Lake CityUSA
  3. 3.Department of BiologyWake Forest UnivesityWinston-SalemUSA

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