Assembly of SRP from Single Polypeptides and 7S RNA
Signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein particle which mediates the targeting of nascent secretory and membrane proteins to the endoplasmic reticulum (ER) by virtue of three activities: (i) The binding to signal sequences once they are exposed on translating ribosomes, (ii) the subsequent slow-down of the elongation until (iii) the binding to the docking protein, the SRP receptor in the membrane of the ER. Upon interacting with the docking protein SRP detaches from the signal sequence and the ribosome. The elongation of the nascent polypeptide resumes and its translocation is initiated. SRP subsequently detaches from the docking protein in a step requiring GTP hydrolysis.
KeywordsSignal Sequence Signal Recognition Particle Docking Protein Upstream Open Reading Frame Signal Recognition Particle Receptor
Unable to display preview. Download preview PDF.
- Lütcke, H., S. High, K. Römisch, A. J. Ashford and B. Dobberstein. (1992a). EMBO J. 11(4): 1543–1551.Google Scholar
- Lütcke, H., S. Prehn, A. J. Ashford, R. Frank and B. Dobberstein. (1992b). submitted.Google Scholar
- Wood, H., J. Luirink. and D. Tollervey. (1992). Nucl. Acids Res: 20 No.22: in the press.Google Scholar