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Towards Ribosomal Structure at Peptide Level: Use of Crosslinking, Antipeptide Antibodies and Limited Proteolysis

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The Translational Apparatus

Abstract

Although remarkable progress has been achieved in the past decades towards an understanding of the structure and function of the ribosome, the interactions of nucleic acids and proteins involved in protein biosynthesis remain largely unresolved. It has convincingly been demonstrated that ribosomal RNA is essential for ribosomal function (e.g. Schulze and Nierhaus, 1982; Dahlberg, 1989; Noller et al., 1992), yet there can be no doubt that in contemporary ribosomes complexes of RNA and proteins constitute the functional units.Models of the tertiary structure of the 16S RNA have been derived from footprinting and crosslinking experiments (Stern et al., 1988; Brimacombe et al., 1988; Nagano et al., 1988). In addition, functionally important domains and even nucleotides were identified by affinity labelling and site-directed mutagenesis.

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Authors and Affiliations

  1. Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Str. 10, O-1115, Berlin-Buch, Germany

    Volker Kruft, Oliver Bischof, Elke Herfurth & Brigitte Wittmann-Liebold

  2. Max-Planck-Institut für Molekulare Genetik, Ihnestr. 73, W-1000, Berlin 33, Germany

    Ulrike Bergmann

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  1. Volker Kruft
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  3. Ulrike Bergmann
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  4. Elke Herfurth
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  1. Max–Planck-Institut für Molekulare Genetik, Berlin, Germany

    Knud H. Nierhaus , François Franceschi  & Alap R. Subramanian ,  & 

  2. Freie Universität Berlin, Berlin, Germany

    Volker A. Erdmann

  3. Max–Delbrück-Centrum für Molekulare Medizin, Berlin, Germany

    Brigitte Wittmann-Liebold

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Kruft, V., Bischof, O., Bergmann, U., Herfurth, E., Wittmann-Liebold, B. (1993). Towards Ribosomal Structure at Peptide Level: Use of Crosslinking, Antipeptide Antibodies and Limited Proteolysis. In: Nierhaus, K.H., Franceschi, F., Subramanian, A.R., Erdmann, V.A., Wittmann-Liebold, B. (eds) The Translational Apparatus. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2407-6_48

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