Effects of pyruvate on pyruvate dehydrogenase kinase of rat heart

  • Tonia C. Carter
  • Haldane G. Coore
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 15)

Abstract

Sensitivity of rat heart pyruvate dehydrogenase kinase (PDHK) to pyruvate inhibition was tested under various conditions using pyruvate dehydrogenase complex (PDC) in mitochondria (mPDC) and in a high speed precipitate of whole tissue homogenates (hPDC). In the latter preparation pyruvate in the range of concentration 1–10 mM caused increasing inhibition of PDHK when the enzyme was prepared from animals fed ad libitum but had no effect when the enzyme was prepared from 48 h starved animals. Similar behaviour was observed in mPDC from fed and starved animals when rotenone was present in the incubation medium. When carbonyl cyanide m-chlorophenylhydrazone (CCCP) instead of rotenone was present, pyruvate at 1 mM concentration stimulated PDHK from hearts of fed animals but was without effect at 10 mM. When mPDC or PDC from hearts of starved animals was incubated at 30°C for 30 min, inhibition of PDHK by pyruvate was restored.

Key words

pyruvate pyruvate dehydrogenase kinase heart 

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Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Tonia C. Carter
    • 1
  • Haldane G. Coore
    • 1
  1. 1.Department of Biochemistry, Faculty of Medical SciencesThe University of the West IndiesSt. AugustineCaribbean

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