Effects of pyruvate on pyruvate dehydrogenase kinase of rat heart

  • Tonia C. Carter
  • Haldane G. Coore
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 15)


Sensitivity of rat heart pyruvate dehydrogenase kinase (PDHK) to pyruvate inhibition was tested under various conditions using pyruvate dehydrogenase complex (PDC) in mitochondria (mPDC) and in a high speed precipitate of whole tissue homogenates (hPDC). In the latter preparation pyruvate in the range of concentration 1–10 mM caused increasing inhibition of PDHK when the enzyme was prepared from animals fed ad libitum but had no effect when the enzyme was prepared from 48 h starved animals. Similar behaviour was observed in mPDC from fed and starved animals when rotenone was present in the incubation medium. When carbonyl cyanide m-chlorophenylhydrazone (CCCP) instead of rotenone was present, pyruvate at 1 mM concentration stimulated PDHK from hearts of fed animals but was without effect at 10 mM. When mPDC or PDC from hearts of starved animals was incubated at 30°C for 30 min, inhibition of PDHK by pyruvate was restored.

Key words

pyruvate pyruvate dehydrogenase kinase heart 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Linn TC, Pettit FH, Reed LJ: Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation. Proc Natl Acad Sci USA 62: 234–241, 1969PubMedCrossRefGoogle Scholar
  2. 2.
    Linn TC, Pettit FH, Hucho F, Reed LJ: Comparative studies of regulatory properties of the pyruvate dehydrogenase complexes from kidney, heart, and liver mitochondria. Proc Natl Acad Sci USA 64: 227–234, 1969PubMedCrossRefGoogle Scholar
  3. 3.
    Yeaman SJ, Hutcheson ET, Roche TE, Pettit FH, Brown JR, Reed LJ, Watson DC, Dixon GH: Sites of phosphorylation on pyruvate dehydrogenase from bovine kidney and heart. Biochemistry 17: 2364–2370, 1978PubMedCrossRefGoogle Scholar
  4. 4.
    Kerbey AL, Randle PJ: Pyruvate dehydrogenase kinase activity of pig heart pyruvate dehydrogenase (E1 component of pyruvate dehydrogenase complex. Biochem J 231: 523–529, 1985PubMedGoogle Scholar
  5. 5.
    Denton RM, Randle PJ, Martin BR: Stimulation by calcium ions of pyruvate dehydrogenase phosphate phosphatase. Biochem J 128: 161–163, 1972PubMedGoogle Scholar
  6. 6.
    Randle PJ: Fuel selection in animals. Biochem SocTrans 14: 799–806, 1986Google Scholar
  7. 7.
    Kerbey AL, Randle PJ, Cooper RH, Whitehouse S, Pask HT, Denton RM: Regulation of pyruvate dehydrogenase in rat heart. Biochem J 154: 327–348, 1976PubMedGoogle Scholar
  8. 8.
    Hutson NJ, Randle PJ: Enhanced activity of pyruvate dehydrogenase kinase in rat heart mitochondria in alloxan-diabetes or starvation. FEBS Lett 92: 73–76, 1978PubMedCrossRefGoogle Scholar
  9. 9.
    Baxter MA, Coore HG: The mode of regulation of pyruvate dehydrogenase of lactating rat mammary gland. Biochem J 174: 553–561, 1978PubMedGoogle Scholar
  10. 10.
    Baxter MA, Coore HG: Starvation of lactating rats leads to alterations in the behaviour of pyruvate dehydrogenase kinase which persist in the semi-purified pyruvate dehydrogenase complex of the mammary gland but are partly reversible in vitro. FEBS Lett 98: 195–198, 1979PubMedCrossRefGoogle Scholar
  11. 11.
    Lyn D, Coore, HG: Pyruvate inhibition of pyruvate dehydrogenase kinase is a physiological variable. Biochem Biophys Res Commun 126: 992–998, 1985PubMedCrossRefGoogle Scholar
  12. 12.
    Cockburn BN, Coore, HG: Starvation reduces pyruvate phosphate phosphatase activity in rat kidney. Mol Cell Biochem, 1995Google Scholar
  13. 13.
    Whitehouse S, Cooper RH, Randle, PJ: Mechanism of activation of pyruvate dehydrogenase by dichloracetate and other halogenated carboxylic acids. Biochem J 141: 761–774, 1974PubMedGoogle Scholar
  14. 14.
    Baxter MA: The regulation, by phosphorylation, of the pyruvate dehydrogenase complex in lactating rat mammary gland [dissertation]. Birmingham, England: University of Birmingham, 1979Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Tonia C. Carter
    • 1
  • Haldane G. Coore
    • 1
  1. 1.Department of Biochemistry, Faculty of Medical SciencesThe University of the West IndiesSt. AugustineCaribbean

Personalised recommendations