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Clonal Expansion of T Cells and HIV Genotypes in Microdissected Splenic White Pulps Indicates Viral Replication in Situ and Infiltration of HIV-Specific Cytotoxic T Lymphocytes

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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 374)

Abstract

Human immunodeficiency virus (HIV) replication and T cell proliferation was investigated in situ by a PCR based analysis of individual microdissected splenic white pulps. Founder effects, revealed by an exquisite compartmentalization of HIV genotypes and T cells, indicated the recruitment of latently infected CD4+ T cells through highly localized antigen presentation, rather than the infection of CD4+ T lymphoblasts by blood borne virus or immune complexes. HIV infected white pulps could be infiltrated by HIV specific cytotoxic T lymphocytes, so implicating them in CD4+ T cell destruction in vivo. Together these data describe an iterative and deleterious mechanism of antigen driven T cell recruitment and activation, HIV replication and spread, with consequent destruction of the newly infected cells.

Keywords

Human Immunodeficiency Virus Human Immunodeficiency Virus Type Human Immunodeficiency Virus Infection White Pulp Human Immunodeficiency Virus Replication 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  1. 1.Unité de Rétrovirologie MoléculaireInstitut PasteurParisFrance
  2. 2.Laboratoire d’Immunologie Cellulaire et TissulaireHôpital La Pitié-SalpétrièreParisFrance
  3. 3.Service d’Immunopathologie et d’HématologieHôpital Saint LouisParisFrance

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