Basement Membrane in the Control of Mammary Gland Function

  • Charles H. Streuli


Milk is produced in the lactating mammary gland by the alveolar epithelium. Alveoli are composed of a single layer of secretory polarized cells that surround a lumen and interact both with one another and with their basement membrane. Caseins and other milk proteins are synthesized and secreted in response to lactogenic hormones. However, milk production also requires cell-cell and cell-extracellular matrix associations. In this article, I discuss how the use of culture models has unravelled different facets of the molecular control of mammary gland differentiation. I show that milk production is dependent on a specific component of the basement membrane and is under coordinate regulation by the matrix as well as hormones.


Basement Membrane Mammary Gland Mammary Epithelial Cell Milk Protein Mouse Mammary Epithelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Aggeler, J., Ward, J., Blackie, L.M., Barcellos-Hoff, M.H., Streuli, C.H. and Bisseil, M.J., 1991, Cytodifferentiation of mouse mammary epithelial cells cultured on a reconstituted basement membrane reveals striking similarities to development in vivo, J. Cell Sci. 99:407.PubMedGoogle Scholar
  2. Barcellos-Hoff, M.H., Aggeler, J., Ram, T.G. and Bisseil, M.J., 1989, Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane, Development 105:223.PubMedGoogle Scholar
  3. Bisseil, M.J., Hall, H.G. and Parry, G., 1982, How does extracellular matrix direct gene expression? J. Theor. Biol. 99:31.CrossRefGoogle Scholar
  4. Burgeson, R.E., Chiquet, M., Deutzmann, R., Ekblom, P., Engel, J., Kleinman, H., Martin, G.R.,Google Scholar
  5. Meneguzzi, G., Paulsson, M., Sanes, J., Timpl, R., Tryggvason, K., Yamada, Y. and Yurchenco, P.D., 1994, A new nomenclature for the laminins, Matrix Biology 14:209.PubMedCrossRefGoogle Scholar
  6. Chammas, R., Taverna, D., Cella, N., Santos, C. and Hynes, N.E., 1994, Laminin and tenascin assembly and expression regulate HC11 mouse mammary cell differentiation, J. Cell Sci. 107:1031.PubMedGoogle Scholar
  7. Darnell, J.E., Kerr, I.M. and Stark, G.R., 1994, Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signalling proteins, Science 264:1415.PubMedCrossRefGoogle Scholar
  8. Delcommenne, M. and Streuli, C.H., Expression of ß1 integrins is regulated by the extracellular matrix, Submitted for publication.Google Scholar
  9. Emerman, J.T., Enami, J., Pitelka, D.R. and Nandi, S., 1977, Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes, Proc. Natl. Acad. Sci. USA 74:4466.PubMedCrossRefGoogle Scholar
  10. Hay, E.D., 1993, Extracellular matrix alters epithelial differentiation, Curr. Opin. Cell Biol. 5:1029.PubMedCrossRefGoogle Scholar
  11. Hildebrand, J.D., Schaller, M.D. and Parsons, J.T., 1993, Identification of sequences required for the efficient localization of the focal adhesion kinase, pp125FAK, to cellular focal contacts, J. Cell Biol. 123:993.PubMedCrossRefGoogle Scholar
  12. Hynes, R.O., 1992, Integrins: versatility, modulation, and signalling in cell adhesion, Cell 69:11.PubMedCrossRefGoogle Scholar
  13. Ingber, D.E., 1993, The riddle of morphogenesis: A question of solution chemistry or molecular cell engineering? Cell 75:1249.PubMedCrossRefGoogle Scholar
  14. Koraberg, L.J., Earp, H.S., Turner, C.E., Prockcop, C. and Juliano, R.L., 1991, Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of ß1 integrins, Proc. Natl. Acad. Sci. 88:8392.CrossRefGoogle Scholar
  15. Lee, E.Y.-H., Parry, G. and Bisseil, M.J., 1984, Modulation of secreted proteins of mouse mammary epithelial cells by the collagenous substrata, J. Cell Biol. 98:146.PubMedCrossRefGoogle Scholar
  16. Li, M.L., Aggeler, J., Farson, D.A., Hatier, C., Hassell, J. and Bissell, M.J., 1987, Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells, Proc. Natl. Acad. Sci. USA 84:136.PubMedCrossRefGoogle Scholar
  17. Reichmann, E., Ball, R., Groner, B. and Friis, R.R., 1989, New mammary epithelial and fibroblastic clones in culture form structures competent to differentiate functionally, J. Cell Biol. 108:1127.PubMedCrossRefGoogle Scholar
  18. Roskelley, C.D., Desprez, P.Y. and Bissell, M.J., 1994, Extracellular matrix-dependent tissue specific gene expression in mammary epithelial cells requires both physical and biochemical signal transduction, Proc. Natl. Acad. Sci. USA, in press.Google Scholar
  19. Rui, H., Kirken, R.A. and Farrar, W.L., 1994, Activation of receptor-associated tyrosine kinase JAK2 by prolactin, J. Biol. Chem. 269:5364.PubMedGoogle Scholar
  20. Schmidhauser, C., Myers, C., Mossi, R., Casperon, G.F. and Bissell, M.J., 1995, Extracellular matrix dependent gene regulation in mammary epithelial cells, in “Intercellular Signalling in the Mammary Gland”, Plenum Publishing Company, London.Google Scholar
  21. Schmidhauser, C., Casperson, G.F., Myers, C.A., Sanzo, K.T., Bolten, S. and Bissell, M.J., 1992, A novel transcriptional enhancer is involved in the prolactin-and extracellular matrix-dependent regulation of beta-casein gene expression, Mol. Biol. Cell. 3:699.PubMedGoogle Scholar
  22. Schmidhauser, C., Bissell, M.J., Myers, C.A. and Casperson, G.F., 1990, Extracellular matrix and hormones transcriptionally regulate bovine beta-casein 5’ sequences in stably transfected mouse mammary cells, Proc. Natl. Acad. Sci. USA 87: 9118.PubMedCrossRefGoogle Scholar
  23. Stoker, A., Streuli, C.H., Martins-Green, M. and Bissell, M.J., 1990, Designer microenvironments for the analysis of cell and tissue function, Curr. Opin. Cell Biol. 2:864.PubMedCrossRefGoogle Scholar
  24. Streuli, C.H. and Bissell, M.J., 1990, Expression of extracellular matrix components is regulated by substratum, J. Cell Biol. 110: 1405.PubMedCrossRefGoogle Scholar
  25. Streuli, C.H., 1993, Extracellular matrix and gene expression in mammary epithelium, Sem. Cell Biol. 4:203.CrossRefGoogle Scholar
  26. Streuli, C.H., Schmidhauser, C., Roskelley, C.D., Bailey, N., Yurchenco, P., Skubitz, A.P.N. and Bissell, M.J., A domain within laminin that mediates tissue-specific gene expression in mammary epithelia, Submitted for publication.Google Scholar
  27. Streuli, C.H., Bailey, N. and Bissell, M.J., 1991, Control of mammary epithelial differentiation: the separate roles of cell-substratum and cell-cell interaction, J. Cell Biol., 115:1383.PubMedCrossRefGoogle Scholar
  28. Sympson, C.J., Talhouk, R.S., Alexander, C.M., Chin, S.K., Clift, S.M., Bissell, M.J. and Werb, Z., 1994, Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression, J. Cell Biol. 125:681.PubMedCrossRefGoogle Scholar
  29. Tomaselli, K.J., Damsky, C.H. and Reichardt, L.F., 1988, Purification and characterization of mammalian integrins expressed by a rat neuronal cell line (PC12): evidence that they function as alpha/beta heterodimeric receptors for laminin and type IV collagen, J. Cell Biol. 107:1241.PubMedCrossRefGoogle Scholar
  30. Wakao, H., Gouilleux, F. and Groner, B., 1994, Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO J.13:2182.PubMedGoogle Scholar
  31. Yurchenco, P.D. and Schittny, J.C., 1990, Molecular architecture of basement membranes. FASEB J. 4:1577.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Charles H. Streuli
    • 1
  1. 1.School of Biological SciencesUniversity of ManchesterManchesterUK

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