Abstract
Mouse mammary epithelial cells (MMEC) from normal glands readily proliferate in culture when plated with lethally irradiated cells of the LA7 rat mammary tumour line (Ehmann et al., 1984). These cells can be sub-cultured at least 12 times (32 cell generations, if fresh feeders are added to the new cultures in numbers large enough to achieve confluency. Cells cultured in this manner appear normal with respect to morphology and dome formation. Their normality is also apparent when introduced into mouse mammary fat pads where they proliferate to form normal mammary ducts (Ehmann et al., 1987).
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References
Ehmann, U.K., 1992, Feeder cells impart a growth stimulus to recipient epithelial cells by direct contact, Exp. Cell Res. 199:314.
Ehmann, U.K., Guzman, R.C., Osborn, R.C., et al. ,1987, Cultured mouse mammary epithelial cells: normal phenotype after implantation, J. Nat. Cancer Inst. 78:751.
Ehmann, U.K., Peterson, W.D., Jr., Misfeldt, D.S., 1984, To grow mouse mammary epithelial cells in culture, J. Cell Biol. 98:1026.
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© 1995 Springer Science+Business Media New York
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Ehmann, U.K., Chen, M.S.C., Adamos, A.A. (1995). Juxtacrine Growth Stimulation of Mouse Mammary Cells in Culture. In: Wilde, C.J., Peaker, M., Knight, C.H. (eds) Intercellular Signalling in the Mammary Gland. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1973-7_13
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DOI: https://doi.org/10.1007/978-1-4615-1973-7_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5812-1
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