Functional Dichotomy of Dendritic Cells Isolated from Blood and Lymph Nodes
Previous studies have shown that dendritic cells (DC) isolated from mouse lymph nodes or spleen can induce primary proliferative responses to contact sensitizers when exposed to the chemical either in vivo or in vitro 1,2 In this study we have attempted to induce primary immune responses to contact sensitizers in vitro using peripheral blood DC. Neither human nor mouse blood DC stimulated a response to picryl sulphonic acid by syngeneic lymphocytes. This contrasted with mouse lymph node or spleen DC where good primary proliferative responses to picryl sulphonic acid have been obtained. Here we have examined adhesion molecule expression on mouse lymph node and blood DC to determine whether functional variation is related to surface molecule expression.
KeywordsFluorescein Dermatitis Thymidine Isothiocyanate Toxicology
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