Functional Dichotomy of Dendritic Cells Isolated from Blood and Lymph Nodes

  • S. Hill
  • J. P. Coates
  • I. Kimber
  • S. C. Knight
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)


Previous studies have shown that dendritic cells (DC) isolated from mouse lymph nodes or spleen can induce primary proliferative responses to contact sensitizers when exposed to the chemical either in vivo or in vitro 1,2 In this study we have attempted to induce primary immune responses to contact sensitizers in vitro using peripheral blood DC. Neither human nor mouse blood DC stimulated a response to picryl sulphonic acid by syngeneic lymphocytes. This contrasted with mouse lymph node or spleen DC where good primary proliferative responses to picryl sulphonic acid have been obtained. Here we have examined adhesion molecule expression on mouse lymph node and blood DC to determine whether functional variation is related to surface molecule expression.


Mouse Lymph Node Primary Immune Response Contact Sensitizer Blood Dendritic Cell Allogeneic Response 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • S. Hill
    • 1
  • J. P. Coates
    • 1
  • I. Kimber
    • 2
  • S. C. Knight
    • 1
  1. 1.Antigen Presentation Research Group, St. Mary’s Hospital Medical SchoolNorthwick Park Institute for Medical ResearchHarrowUK
  2. 2.Zeneca Central Toxicology LaboratoryMacclesfield, CheshireUK

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