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Purification of Human Lung Dendritic Cells by Three Color Flow Cytometry

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Book cover Dendritic Cells in Fundamental and Clinical Immunology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 378))

Abstract

Dendritic cells (DC) are potent Antigen Presenting Cells (APC) distributed among many tissues, including non lymphoid organs: epidermis, heart, liver, lung and gut and lymphoid organs: blood, lymph, tonsil, thymus (1). DC can present antigen to T cells and then induce strong T cell-mediated immune responses (2).

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References

  1. R.M. Steinman. The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol. 9:271 (1991).

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  2. C. Guidos, M. Wong, and K.C. Lee. A comparison of the stimulating activities of lymphoid dendritic cells and macrophages in T proliferative responses to various antigens. J. Immunol. 133:1179 (1984).

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  3. L.P. Nicod, M.F. Lipscomb, J.C. Weissler, and G.B. Toews. Mononuclear cells from human lung parenchyma support antigen-induced T lymphocyte proliferation. J. Leuk. Biol 45:336 (1989).

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  4. R. Thomas, L.S. Davis, and P.E. Lipsky. Comparative accessory cell function of human peripheral blood dendritic cells and monocytes. J. Immunol. 151:6840 (1993).

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Authors and Affiliations

  1. Glaxo Institute for Molecular Biology, 14 chemin des Aulx, 1228, Plan les Ouates / Geneva, Switzerland

    Jean-Pierre Aubry, Christine Power, Paul Life & Jean-Yves Bonnefoy

  2. Centre Médical Universitaire, Geneva, Switzerland

    Magdalena Schrader

Authors
  1. Jean-Pierre Aubry
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  2. Christine Power
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  3. Paul Life
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  4. Magdalena Schrader
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  5. Jean-Yves Bonnefoy
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Editor information

Editors and Affiliations

  1. Laboratory for Immunological Research, Schering-Plough, Dardilly, France

    Jacques Banchereau

  2. INSERM U346, Hôpital Edouard Herriot, Lyon, France

    Daniel Schmitt

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© 1995 Springer Science+Business Media New York

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Aubry, JP., Power, C., Life, P., Schrader, M., Bonnefoy, JY. (1995). Purification of Human Lung Dendritic Cells by Three Color Flow Cytometry. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_18

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  • DOI: https://doi.org/10.1007/978-1-4615-1971-3_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5811-4

  • Online ISBN: 978-1-4615-1971-3

  • eBook Packages: Springer Book Archive

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