Dendritic Cells Differentiated from Human Monocytes through a Combination of IL-4, GM-CSF and IFN-γ Exhibit Phenotype and Function of Blood Dendritic Cells

  • Hui Xu
  • Manuela Kramer
  • Hans.-P. Spengler
  • J. Hinrich Peters
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)

Summary

Our previous studies demonstrated that monocytes, when cultured under certain conditions, are able to differentiate into DC-like cells (MoDC) presenting a high accessory activity and low phagocytic function. In the present study, we demonstrate that under the effect of a triple combination of IL-4, IFN-γ and GM-CSF human blood monocytes are able to differentiate into the cells expressing an identical phenotype and functional features of blood dendritic cells. MoDC stimulated T cell proliferation 20 –30 times higher than untreated monocytes, similar to blood DC. They expressed abundant HLA-DR molecules, but only trace amounts of the monocyte/macrophage markers CD16 (FcR III), CD32 (FcR II), and CD14. Phagocytosis of Ig- and complement-opsonized bacteria was reduced by 93%.

Keywords

Monophosphat Lost 

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References

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Hui Xu
    • 1
  • Manuela Kramer
    • 2
  • Hans.-P. Spengler
  • J. Hinrich Peters
    • 3
  1. 1.Dept. of Immunology, Research Inst. NNECleveland Clinic FoundationClevelandUSA
  2. 2.Dermatology Hospital, Klinikum InnenstadtUniversity of MunchenMunchenGermany
  3. 3.Dept. of ImmunologyUniversity of GottingenGöttingenGermany

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