Dendritic Cells Differentiated from Human Monocytes through a Combination of IL-4, GM-CSF and IFN-γ Exhibit Phenotype and Function of Blood Dendritic Cells

  • Hui Xu
  • Manuela Kramer
  • Hans.-P. Spengler
  • J. Hinrich Peters
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)


Our previous studies demonstrated that monocytes, when cultured under certain conditions, are able to differentiate into DC-like cells (MoDC) presenting a high accessory activity and low phagocytic function. In the present study, we demonstrate that under the effect of a triple combination of IL-4, IFN-γ and GM-CSF human blood monocytes are able to differentiate into the cells expressing an identical phenotype and functional features of blood dendritic cells. MoDC stimulated T cell proliferation 20 –30 times higher than untreated monocytes, similar to blood DC. They expressed abundant HLA-DR molecules, but only trace amounts of the monocyte/macrophage markers CD16 (FcR III), CD32 (FcR II), and CD14. Phagocytosis of Ig- and complement-opsonized bacteria was reduced by 93%.


Relative Fluorescence Intensity Triple Combination Complete Abrogation CDl4 Expression Identical Phenotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R.K.H. Gieseler, R-A. Röber, R. Kuhn, W. Weber, M. Osborn, and J.H. Peters: Dendritic cells (DC) derived from rat bone marrow precursors under defined conditions invitro belong to the myeloid lineage. Eur. J. Cell Biol. (1991) 54:171–181.PubMedGoogle Scholar
  2. 2.
    J.H. Peters, S. Ruhl, D. Friedrichs: Veiled accessory cells deduced from monocytes.Immunobiol. (1987) 176:154–166.CrossRefGoogle Scholar
  3. 3.
    J.H. Peters, T. Börner, J. Ruppert: Accessory phenotype and function of macrophagesinduced by cyclic adenosine monophosphat. Int. Immunol. (1990) 2:1195–1202.PubMedCrossRefGoogle Scholar
  4. 4.
    J.H. Peters, H. Xu, J. Ruppert, D. Ostermeier, D. Friedrichs, and RICH. Gieseler: Signals required for differentiating dendritic cells from human monocytes in vitro. In: E.W.A. Kamperdijk, E.C.M. Hoefsmit, P. Nieuwenhuis, (Eds.) Dendritic cells in Fundamental and Clinical Immunology. Plenum Press 1993, 275–280.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Hui Xu
    • 1
  • Manuela Kramer
    • 2
  • Hans.-P. Spengler
  • J. Hinrich Peters
    • 3
  1. 1.Dept. of Immunology, Research Inst. NNECleveland Clinic FoundationClevelandUSA
  2. 2.Dermatology Hospital, Klinikum InnenstadtUniversity of MunchenMunchenGermany
  3. 3.Dept. of ImmunologyUniversity of GottingenGöttingenGermany

Personalised recommendations