Rosettes of Langerhans Cells and Activated T Cells in Human Skin Lymph Derived from Irritant Contact Dermatitis
In addition to its role as a mechanical and physicochemical barrier the skin acts as an immunological control and defence system. Thereby, immunological surveillance is principally performed by circulating cells in concert with soluble mediators. In this context the lymphatic system plays a major role, as its vessels allow the clearance of proteins and fluid from the tissues and provide the exit pathway for immunocompetent cells. Starting in the papillary dermis, the skin lymphatics drain the adjacent tissue in a one-way flow towards the regional lymph nodes. Thus, the composition of the afferent lymph reflects inflammatory processes in the drained tissue. Today, in human probands, it is possible to collect afferent lymph exclusively derived from a selected skin area1. By inducing specific skin lesions in the drained skin area, this system of lymph cannulation allows to get insight into the local pathomechanisms of skin disorders. In contrast to studies taking single skin biopsies of certain time-points of an experiment, the kinetics of skin processes can be assessed continuously.
KeywordsContact Dermatitis Sodium Lauryl Sulphate Allergic Contact Dermatitis Papillary Dermis Irritant Contact Dermatitis
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