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Rosettes of Langerhans Cells and Activated T Cells in Human Skin Lymph Derived from Irritant Contact Dermatitis

  • Ch. U. Brand
  • Th. Hunziker
  • H. A. Gerber
  • Th. Schaffner
  • A. Limat
  • L. R. Braathen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)

Abstract

In addition to its role as a mechanical and physicochemical barrier the skin acts as an immunological control and defence system. Thereby, immunological surveillance is principally performed by circulating cells in concert with soluble mediators. In this context the lymphatic system plays a major role, as its vessels allow the clearance of proteins and fluid from the tissues and provide the exit pathway for immunocompetent cells. Starting in the papillary dermis, the skin lymphatics drain the adjacent tissue in a one-way flow towards the regional lymph nodes. Thus, the composition of the afferent lymph reflects inflammatory processes in the drained tissue. Today, in human probands, it is possible to collect afferent lymph exclusively derived from a selected skin area1. By inducing specific skin lesions in the drained skin area, this system of lymph cannulation allows to get insight into the local pathomechanisms of skin disorders. In contrast to studies taking single skin biopsies of certain time-points of an experiment, the kinetics of skin processes can be assessed continuously.

Keywords

Contact Dermatitis Sodium Lauryl Sulphate Allergic Contact Dermatitis Papillary Dermis Irritant Contact Dermatitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Brand CU, Hunziker T, Braathen LR: Isolation of human skin-derived lymph. Arch Dermatol Res 1992; 284:123–126PubMedCrossRefGoogle Scholar
  2. 2.
    Reitamo S, Tolvanen E, Konttinen YT, Käyhkö K, Förström L, Salo OP. Allergic and toxic contact dermatitis: Inflammatory cell subtypes in epicutaneous test reactions. Brit J Dermatol 1981; 105: 521–527CrossRefGoogle Scholar
  3. 3.
    Scheynius A, Fischer T, Forsum U, Klareskog L. Phenotypic characterization in situ of inflammatory cells in allergic and irritant contact dermatitis in man. Clin Exp Immunol 1984; 55: 81–90Google Scholar
  4. 4.
    Ranki A, Kanerva L, Förstrom L, Konttinen YT, Mustakallio KK. T and B lymphocytes, macrophages and Langerhans cells during the course of contact allergic and irritant skin reactions in man. Acta Derm Venereol 1983; 63: 376–383Google Scholar
  5. 5.
    Willis CM, Young E, Brandon DR, Wilkinson JD. Immunopathological and ultrastructural findings in human allergic and irritant contact dermatitis. Brit J Dermatol 1986; 115: 305–316CrossRefGoogle Scholar
  6. 6.
    Kanerva L, Ranki A, Lauharanta J. Lymphocytes and Langerhans cells in patch tests. Contact Dermatitis 1984; 11: 150–155PubMedCrossRefGoogle Scholar
  7. 7.
    Christensen OB, Wall LM. Long term effect on epidermal dendritic cells of four different types of exogenous inflammation. Acta Derm Venereol 1987; 67: 305–309PubMedGoogle Scholar
  8. 8.
    Scheynius A, Dalenbring M, Carlsson K, England R, Lindberg M: Quantitative analysis of Langerhans cells in epidermis at irritant contact reactions using confocal laser scanning microscopy. Acta Derm Venereol 1992;72:348–351PubMedGoogle Scholar
  9. 9.
    Mikulowska A. Reactive changes in the Langerhans cells of human skin caused by occlusion with water and sodium lauryl sulphate. Acta Derm Venereol 1990; 70: 468–473PubMedGoogle Scholar
  10. 10.
    Brand CU, Hunziker T, Braathen LR: Studies on human skin lymph containing Langerhans cells from sodium lauryl sulphate contact dermatitis. J Invest Dermatol 1992; 99: 109S–110SPubMedCrossRefGoogle Scholar
  11. 11.
    Brand CU, Hunziker T, Limat A, Braathen LR: Large increase of Langerhans cells in human skin lymph derived from irritant contact dermatitis. Brit J Dermatol 1993; 128: 184–188CrossRefGoogle Scholar
  12. 12.
    Brand CU, Hunziker T, Schaffner Th, Limat A, Gerber H, Braathen LR: Activated immunocompetent cells in human skin lymph derived from irritant contact dermatitis: An immunomorphological study. Brit J Dermatol (in press)Google Scholar
  13. 13.
    Fujita M, Miyachi Y, Nakata K, Imamura S: γ T-cell receptor-positive cells of human skin. II.Appearance in delayed-type hypersensitivity reaction. Arch Dermatol Res 1993; 285: 436–440PubMedCrossRefGoogle Scholar
  14. 14.
    Hunziker T, Brand CU, Limat A, Braathen LR: Alloactivating and antigen-presenting capacities of human skin lymph cells derived from sodium lauryl sulphate-induced contact dermatitis. European J Dermatol 1993: 3: 137–140Google Scholar
  15. 15.
    Hunziker T, Brand CU, Kapp A, Wälti ER, Braathen LR: Increased levels of inflammatory cytokines in human skin lymph derived from irritant contact dermatitis. Brit J Dermatol 1992: 127: 254–257CrossRefGoogle Scholar
  16. 16.
    Brand CU, Späth PJ, Hunziker T, Limat A, Braathen LR: Complement Profiles in Human Skin Lymph in the Course of Irritant Contact Dermatitis. Arch Derm Res (in press)Google Scholar
  17. 17.
    Bos JD, Kapsenberg ML: The skin immune system: progress in cutaneous biology. Immunol. Today 1993; 14: 75–78PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Ch. U. Brand
    • 1
  • Th. Hunziker
    • 1
  • H. A. Gerber
    • 2
  • Th. Schaffner
    • 2
  • A. Limat
    • 1
  • L. R. Braathen
    • 1
  1. 1.Dermatological ClinicUniversity of BerneBernSwitzerland
  2. 2.Institute of PathologyUniversity of BerneBernSwitzerland

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