In Vitro HIV-1 Infection of Isolated Epidermal Langerhans Cells with a Cell-Free System
Langerhans cells (LC) belong to the antigen-presenting cells which are found in all lymphoid and non-lymphoid organs (1). Originating from the bone marrow the LC migrate to the peripheral epithelia (skin, mucous membranes) where they play a key role in the immune surveillance against foreign antigens then migrate to lymph nodes. LC are localized in areas at risk for inoculation by Human Immunodeficiency Virus (HIV). In seropositive patients, LC are susceptible to be targets for HIV (for review, see 2) that it had been shown by a large variety of methodologies such as electron microscopy, immunocytochemistry, polymerase chain reaction (PCR) amplification of virus-specific DNA, reverse transcription of HIV-1 spliced mRNA region of the tat gene (3). In a coculture with HIV-1-carrying U937 cells, we had demonstrated the infectiousness of LC isolated from human normal skin in vitro which supported replication (4). The aim of this study was to investigate whether LC can be infected in vitro by HIV-1 provided by a cell-free infection system.
KeywordsMigration Filtration Trypsin
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