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In Vitro HIV-1 Infection of Isolated Epidermal Langerhans Cells with a Cell-Free System

  • Anne-Sophie Charbonnier
  • François Mallet
  • Marie-Madeleine Fiers
  • Claude Desgranges
  • Colette Dezutter-Dambuyant
  • Daniel Schmitt
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 378)

Abstract

Langerhans cells (LC) belong to the antigen-presenting cells which are found in all lymphoid and non-lymphoid organs (1). Originating from the bone marrow the LC migrate to the peripheral epithelia (skin, mucous membranes) where they play a key role in the immune surveillance against foreign antigens then migrate to lymph nodes. LC are localized in areas at risk for inoculation by Human Immunodeficiency Virus (HIV). In seropositive patients, LC are susceptible to be targets for HIV (for review, see 2) that it had been shown by a large variety of methodologies such as electron microscopy, immunocytochemistry, polymerase chain reaction (PCR) amplification of virus-specific DNA, reverse transcription of HIV-1 spliced mRNA region of the tat gene (3). In a coculture with HIV-1-carrying U937 cells, we had demonstrated the infectiousness of LC isolated from human normal skin in vitro which supported replication (4). The aim of this study was to investigate whether LC can be infected in vitro by HIV-1 provided by a cell-free infection system.

Keywords

Human Immunodeficiency Virus Human Immunodeficiency Virus Type U937 Cell Reverse Transcriptase Activity Dermal Dendritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    M. D. Schuler. “Epidemal Langerhans Cells”, CRC Press, Boston.Google Scholar
  2. 2.
    C. Dezutter-Dambuyant, and D. Schmitt, Epidermal Langerhans cells and HIV-1 infection,Immunol. Lett. ,39: 33 (1993).Google Scholar
  3. 3.
    A. Giannetti, G. Zambruno, A. Cimarelli, A. Marconi, M. Negroni, G. Girolomoni, and Bertazzoni U., Direct detection of HIV-1 RNA in epithelial langerhans cells of HIV-infected patients, J. Acq. Immun. Deflc. Syndrome 6: 329 (1993).Google Scholar
  4. 4.
    P. Delorme, C. Dezutter-Dambuyant, A. Ebersold, C. Desgranges, J. Thivolet, and D.Schmitt, In vitro infection of epidermal Langerhans cells with human immunodeficiency virus type 1 (HTLV-IIIB isolate), Res. Virol. 144: 53 (1993).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Anne-Sophie Charbonnier
    • 1
  • François Mallet
    • 2
  • Marie-Madeleine Fiers
    • 1
  • Claude Desgranges
    • 3
  • Colette Dezutter-Dambuyant
    • 1
  • Daniel Schmitt
    • 1
  1. 1.Unité INSERM 346, Clinique Dermatologique, Pavilion RHopital Edouard HerriotLyon cedex 03France
  2. 2.Unité Mixte CNRS/bioMérieuxEcole Normale Supérieure de LyonLyonFrance
  3. 3.Unité INSERM 271LyonFrance

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